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[[Image:1o7z.jpg|left|200px]]<br /><applet load="1o7z" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1o7z, resolution 1.92&Aring;" />
'''CRYSTAL STRUCTURE OF IP-10 T-FORM'''<br />


==Overview==
==Crystal structure of IP-10 T-form==
We have determined the structure of wild-type IP-10 from three crystal, forms. The crystals provide eight separate models of the IP-10 chain, all, differing substantially from a monomeric IP-10 variant examined previously, by NMR spectroscopy. In each crystal form, IP-10 chains form conventional, beta sheet dimers, which, in turn, form a distinct tetrameric assembly., The M form tetramer is reminiscent of platelet factor 4, whereas the T and, H forms feature a novel twelve-stranded beta sheet. Analytical, ultracentrifugation indicates that, in free solution, IP-10 exists in a, monomer-dimer equilibrium with a dissociation constant of 9 microM. We, propose that the tetrameric structures may represent species promoted by, the binding of glycosaminoglycans. The binding sites for several, IP-10-neutralizing mAbs have also been mapped.
<StructureSection load='1o7z' size='340' side='right'caption='[[1o7z]], [[Resolution|resolution]] 1.92&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1o7z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1O7Z FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.92&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1o7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7z OCA], [https://pdbe.org/1o7z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1o7z RCSB], [https://www.ebi.ac.uk/pdbsum/1o7z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1o7z ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CXL10_HUMAN CXL10_HUMAN] Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o7/1o7z_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1o7z ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We have determined the structure of wild-type IP-10 from three crystal forms. The crystals provide eight separate models of the IP-10 chain, all differing substantially from a monomeric IP-10 variant examined previously by NMR spectroscopy. In each crystal form, IP-10 chains form conventional beta sheet dimers, which, in turn, form a distinct tetrameric assembly. The M form tetramer is reminiscent of platelet factor 4, whereas the T and H forms feature a novel twelve-stranded beta sheet. Analytical ultracentrifugation indicates that, in free solution, IP-10 exists in a monomer-dimer equilibrium with a dissociation constant of 9 microM. We propose that the tetrameric structures may represent species promoted by the binding of glycosaminoglycans. The binding sites for several IP-10-neutralizing mAbs have also been mapped.


==About this Structure==
Crystal structures of oligomeric forms of the IP-10/CXCL10 chemokine.,Swaminathan GJ, Holloway DE, Colvin RA, Campanella GK, Papageorgiou AC, Luster AD, Acharya KR Structure. 2003 May;11(5):521-32. PMID:12737818<ref>PMID:12737818</ref>
1O7Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7Z OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Crystal structures of oligomeric forms of the IP-10/CXCL10 chemokine., Swaminathan GJ, Holloway DE, Colvin RA, Campanella GK, Papageorgiou AC, Luster AD, Acharya KR, Structure. 2003 May;11(5):521-32. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12737818 12737818]
</div>
[[Category: Single protein]]
<div class="pdbe-citations 1o7z" style="background-color:#fffaf0;"></div>
[[Category: Acharya, K.R.]]
[[Category: Holloway, D.E.]]
[[Category: Papageorgiou, A.C.]]
[[Category: Swaminathan, G.J.]]
[[Category: chemokine]]
[[Category: chemotaxis]]
[[Category: inflammatory response]]
[[Category: interferon induction]]


''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:33:19 2008''
==See Also==
*[[C-X-C motif chemokine 3D structures|C-X-C motif chemokine 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Acharya KR]]
[[Category: Holloway DE]]
[[Category: Papageorgiou AC]]
[[Category: Swaminathan GJ]]

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