4apb: Difference between revisions

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-[[Image:4apb.jpg|left|200px]]
-<!--
+==Crystal structure of Mycobacterium tuberculosis fumarase (Rv1098c) S318C in complex with fumarate==
-The line below this paragraph, containing "STRUCTURE_4apb", creates the "Structure Box" on the page.
+<StructureSection load='4apb' size='340' side='right'caption='[[4apb]], [[Resolution|resolution]] 1.94&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[4apb]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4APB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4APB FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.94&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FUM:FUMARIC+ACID'>FUM</scene></td></tr>
-{{STRUCTURE_4apb|  PDB=4apb  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4apb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4apb OCA], [https://pdbe.org/4apb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4apb RCSB], [https://www.ebi.ac.uk/pdbsum/4apb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4apb ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/FUMC_MYCTU FUMC_MYCTU] Catalyzes the reversible addition of water to fumarate to give L-malate.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+rv1098c, an essential gene in Mycobacterium tuberculosis, codes for a class II fumarase. We describe here the crystal structure of Rv1098c in complex with l-malate, fumarate or the competitive inhibitor meso-tartrate. The models reveal that substrate binding promotes the closure of the active site through conformational changes involving the catalytic SS-loop and the C-terminal domain, which likely represents a general feature of this enzyme superfamily. Analysis of ligand-enzyme interactions as well as site-directed mutagenesis suggest Ser318 as one of the two acid-base catalysts. STRUCTURED SUMMARY OF PROTEIN INTERACTIONS: Rv1098c and Rv1098c bind by X-ray crystallography (View interaction).
-===Crystal structure of Mycobacterium tuberculosis fumarase (Rv1098c) S318C in complex with fumarate===
+Conformational changes upon ligand binding in the essential class II fumarase Rv1098c from Mycobacterium tuberculosis.,Mechaly AE, Haouz A, Miras I, Barilone N, Weber P, Shepard W, Alzari PM, Bellinzoni M FEBS Lett. 2012 Jun 4;586(11):1606-11. Epub 2012 May 3. PMID:22561013<ref>PMID:22561013</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 4apb" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-[[4apb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4APB OCA].
+*[[Fumarase|Fumarase]]
-[[Category: Fumarate hydratase]]
+== References ==
-[[Category: Mycobacterium tuberculosis]]
+<references/>
-[[Category: Alzari, P M.]]
+__TOC__
-[[Category: Bellinzoni, M.]]
+</StructureSection>
-[[Category: Haouz, A.]]
+[[Category: Large Structures]]
-[[Category: Mechaly, A E.]]
+[[Category: Mycobacterium tuberculosis H37Rv]]
-[[Category: Hydrolase]]
+[[Category: Alzari PM]]
-[[Category: Metabolism]]
+[[Category: Bellinzoni M]]
+[[Category: Haouz A]]
+[[Category: Mechaly AE]]