4eq8: Difference between revisions

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-'''Unreleased structure'''
-The entry 4eq8 is ON HOLD
+==Crystal structure of PA1844 from Pseudomonas aeruginosa PAO1==
+<StructureSection load='4eq8' size='340' side='right'caption='[[4eq8]], [[Resolution|resolution]] 1.39&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4eq8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EQ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EQ8 FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eq8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eq8 OCA], [https://pdbe.org/4eq8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eq8 RCSB], [https://www.ebi.ac.uk/pdbsum/4eq8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eq8 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/TSE1_PSEAE TSE1_PSEAE] Toxin secreted by the H1 type VI (H1-T6SS) secretion system into the periplasm of recipient cells. Degrades peptidoglycan via amidase activity thereby helping itself to compete with other bacteria (PubMed:21776080, PubMed:22931054, PubMed:22813741, PubMed:22700987). To protect itself, the bacterium synthesizes immunity protein Tsi1 that specifically interacts with and inactivates cognate toxin (PubMed:21776080, PubMed:22931054, PubMed:22700987).<ref>PMID:21776080</ref> <ref>PMID:22700987</ref> <ref>PMID:22813741</ref> <ref>PMID:22931054</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Tse1, an effector protein produced by Pseudomonas aeruginosa, is an amidase hydrolyzing gamma-D-glutamyl-L-meso-diaminopimelic acid (gamma-D-glutamyl-DAP) linkage of the peptide bridge of peptidoglycan. P. aeruginosa injects Tse1 into the periplasm of recipient cells, degrading their peptidoglycan, thereby helping itself to compete with other bacteria. Meanwhile, to protect itself from injury by Tse1, P. aeruginosa expresses the cognate immunity protein Tsi1 in its own periplasm to inactivate Tse1. In the present study, we report the crystal structures of Tse1 and Tse1 (6-148)-Tsi1 (20-end) complex at 1.4 A and 1.6 A resolutions, respectively. The Tse1 structure adopts a classical papain-like alpha+beta fold. A Cys-His catalytic diad is identified in the reaction centre of Tse1 by structural comparison and mutagenesis studies. Tsi1 binds Tse1 tightly. The HI loop (middle finger tip) from Tsi1 inserts into the large pocket of Y-shaped groove on the surface of Tse1, and CD, EF, JK and LM loops (thumb, index finger, ring finger and little finger tips) interact with Tse1, thus blocking the binding of enzyme to peptidoglycan. The catalytic and inhibition mechanisms give new insights into how P. aeruginosa competes with others and protects itself..
-Authors: Shang, G., Li, N., Zhang, J., Lu, D., Yu, Q., Zhao, Y., Liu, X., Xu, S., Gu, L.
+Structural insight into functioning of Pseudomonas aeruginosa peptidoglycan-hydolase Tse1 and its immunity protein Tsi1.,Shang G, Liu X, Lu D, Zhang J, Li N, Zhu C, Liu S, Yu Q, Zhao Y, Zhang H, Hu J, Cang H, Xu S, Gu L Biochem J. 2012 Aug 29. PMID:22931054<ref>PMID:22931054</ref>
-Description: Crystal structure of PA1844 from Pseudomonas aeruginosa PAO1
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 4eq8" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Pseudomonas aeruginosa PAO1]]
+[[Category: Gu L]]
+[[Category: Li N]]
+[[Category: Liu X]]
+[[Category: Lu D]]
+[[Category: Shang G]]
+[[Category: Xu S]]
+[[Category: Yu Q]]
+[[Category: Zhang J]]
+[[Category: Zhao Y]]