2ye1: Difference between revisions

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-'''Unreleased structure'''
-The entry 2ye1 is ON HOLD  until Paper Publication
+==X-ray structure of the cyan fluorescent proteinmTurquoise-GL (K206A mutant)==
+<StructureSection load='2ye1' size='340' side='right'caption='[[2ye1]], [[Resolution|resolution]] 1.63&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2ye1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YE1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YE1 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.63&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SWG:2-[(4Z)-2-[(1R)-1-AMINO-2-HYDROXY-ETHYL]-4-(1H-INDOL-3-YLMETHYLIDENE)-5-OXO-IMIDAZOL-1-YL]ETHANOIC+ACID'>SWG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ye1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ye1 OCA], [https://pdbe.org/2ye1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ye1 RCSB], [https://www.ebi.ac.uk/pdbsum/2ye1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ye1 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Optimization of autofluorescent proteins by intensity-based screening of bacteria does not necessarily identify the brightest variant for eukaryotes. We report a strategy to screen excited state lifetimes, which identified cyan fluorescent proteins with long fluorescence lifetimes (&gt;3.7 ns) and high quantum yields (&gt;0.8). One variant, mTurquoise, was 1.5-fold brighter than mCerulean in mammalian cells and decayed mono-exponentially, making it an excellent fluorescence resonance energy transfer (FRET) donor.
-Authors: von Stetten, D., Noirclerc-Savoye, M., Goedhart, J., Gadella, T.W.J., Royant, A.
+Bright cyan fluorescent protein variants identified by fluorescence lifetime screening.,Goedhart J, van Weeren L, Hink MA, Vischer NO, Jalink K, Gadella TW Jr Nat Methods. 2010 Feb;7(2):137-9. Epub 2010 Jan 17. PMID:020081836<ref>PMID:020081836</ref>
-Description: X-ray structure of the cyan fluorescent proteinmTurquoise-GL (K206A mutant)
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2ye1" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Aequorea victoria]]
+[[Category: Large Structures]]
+[[Category: Gadella TWJ]]
+[[Category: Goedhart J]]
+[[Category: Noirclerc-Savoye M]]
+[[Category: Royant A]]
+[[Category: Von Stetten D]]