2lfr: Difference between revisions

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-[[Image:2lfr.png|left|200px]]
-<!--
+==Solution structure of the chimeric Af1503 HAMP- EnvZ DHp homodimer==
-The line below this paragraph, containing "STRUCTURE_2lfr", creates the "Structure Box" on the page.
+<StructureSection load='2lfr' size='340' side='right'caption='[[2lfr]]' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2lfr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_DSM_4304 Archaeoglobus fulgidus DSM 4304] and [https://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LFR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LFR FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lfr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lfr OCA], [https://pdbe.org/2lfr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lfr RCSB], [https://www.ebi.ac.uk/pdbsum/2lfr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lfr ProSAT]</span></td></tr>
-{{STRUCTURE_2lfr|  PDB=2lfr  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/ENVZ_SHIFL ENVZ_SHIFL] Member of the two-component regulatory system EnvZ/OmpR involved in the regulation of osmoregulation (genes ompF and ompC). EnvZ functions as a membrane-associated protein kinase that phosphorylates OmpR in response to environmental signals (By similarity). This two-component system plays a role in virulence (By similarity).[UniProtKB:A0A4P7TSF2][UniProtKB:P0AEJ4][https://www.uniprot.org/uniprot/O28769_ARCFU O28769_ARCFU]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Bacterial transmembrane receptors regulate an intracellular catalytic output in response to extracellular sensory input. To investigate the conformational changes that relay the regulatory signal, we have studied the HAMP domain, a ubiquitous intracellular module connecting input to output domains. HAMP forms a parallel, dimeric, four-helical coiled coil, and rational substitutions in our model domain (Af1503 HAMP) induce a transition in its interhelical packing, characterized by axial rotation of all four helices (the gearbox signaling model). We now illustrate how these conformational changes are propagated to a downstream domain by fusing Af1503 HAMP variants to the DHp domain of EnvZ, a bacterial histidine kinase. Structures of wild-type and mutant constructs are correlated with ligand response in vivo, clearly associating them with distinct signaling states. We propose that altered recognition of the catalytic domain by DHp, rather than a shift in position of the phospho-accepting histidine, forms the basis for regulation of kinase activity.
-===Solution structure of the chimeric Af1503 HAMP- EnvZ DHp homodimer===
+Mechanism of regulation of receptor histidine kinases.,Ferris HU, Dunin-Horkawicz S, Hornig N, Hulko M, Martin J, Schultz JE, Zeth K, Lupas AN, Coles M Structure. 2012 Jan 11;20(1):56-66. PMID:22244755<ref>PMID:22244755</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_22244755}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 2lfr" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 22244755 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_22244755}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Archaeoglobus fulgidus DSM 4304]]
-[[2lfr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus,_shigella_flexneri Archaeoglobus fulgidus, shigella flexneri]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LFR OCA].
+[[Category: Large Structures]]
+[[Category: Shigella flexneri]]
-==Reference==
+[[Category: Coles M]]
-<ref group="xtra">PMID:022244755</ref><references group="xtra"/>
+[[Category: Ferris HU]]
-[[Category: Archaeoglobus fulgidus, shigella flexneri]]
+[[Category: Hulko M]]
-[[Category: Histidine kinase]]
+[[Category: Lupas AN]]
-[[Category: Coles, M.]]
+[[Category: Martin J]]
-[[Category: Ferris, H U.]]
-[[Category: Hulko, M.]]
-[[Category: Lupas, A N.]]
-[[Category: Martin, J.]]
-[[Category: Gearbox model]]
-[[Category: Hamp domain]]
-[[Category: Histidine kinase]]
-[[Category: Transferase]]
-[[Category: Transmembrane signaling]]