2le3: Difference between revisions

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-[[Image:2le3.png|left|200px]]
-<!--
+==N-terminal regulatory segment of carnitine palmitoyltransferase 1A==
-The line below this paragraph, containing "STRUCTURE_2le3", creates the "Structure Box" on the page.
+<StructureSection load='2le3' size='340' side='right'caption='[[2le3]]' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2le3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LE3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LE3 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2le3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2le3 OCA], [https://pdbe.org/2le3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2le3 RCSB], [https://www.ebi.ac.uk/pdbsum/2le3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2le3 ProSAT]</span></td></tr>
-{{STRUCTURE_2le3|  PDB=2le3  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/CPT1A_HUMAN CPT1A_HUMAN]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The enzyme carnitine palmitoyltransferase 1 (CPT1), which is anchored in the outer mitochondrial membrane (OMM), controls the rate-limiting step in fatty acid beta-oxidation in mammalian tissues. It is inhibited by malonyl-CoA, the first intermediate of fatty acid synthesis, and it responds to OMM curvature and lipid characteristics, which reflect long term nutrient/hormone availability. Here, we show that the N-terminal regulatory domain (N) of CPT1A can adopt two complex amphiphilic structural states, termed Nalpha and Nbeta, that interchange in a switch-like manner in response to offered binding surface curvature. Structure-based site-directed mutageneses of native CPT1A suggest Nalpha to be inhibitory and Nbeta to be noninhibitory, with the relative Nalpha/Nbeta ratio setting the prevalent malonyl-CoA sensitivity of the enzyme. Based on the amphiphilic nature of N and molecular modeling, we propose malonyl-CoA sensitivity to be coupled to the properties of the OMM by Nalpha-OMM associations that alter the Nalpha/Nbeta ratio. For enzymes residing at the membrane-water interface, this constitutes an integrative regulatory mechanism of exceptional sophistication.
-===N-terminal regulatory segment of carnitine palmitoyltransferase 1A===
+An Environment-dependent Structural Switch Underlies the Regulation of Carnitine Palmitoyltransferase 1A.,Rao JN, Warren GZ, Estolt-Povedano S, Zammit VA, Ulmer TS J Biol Chem. 2011 Dec 9;286(49):42545-54. Epub 2011 Oct 11. PMID:21990363<ref>PMID:21990363</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2le3" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_21990363}}, adds the Publication Abstract to the page
+*[[Carnitine palmitoyltransferase|Carnitine palmitoyltransferase]]
-(as it appears on PubMed at http://www.pubmed.gov), where 21990363 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_21990363}}
+__TOC__
+</StructureSection>
-==About this Structure==
-[[2le3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LE3 OCA].
-==Reference==
-<ref group="xtra">PMID:021990363</ref><references group="xtra"/>
-[[Category: Carnitine O-palmitoyltransferase]]
 [[Category: Homo sapiens]]
-[[Category: Rao, J N.]]
+[[Category: Large Structures]]
-[[Category: Ulmer, T S.]]
+[[Category: Rao JN]]
-[[Category: Amphiphilic structure]]
+[[Category: Ulmer TS]]
-[[Category: Membrane protein]]
-[[Category: Membrane-protein interaction]]
-[[Category: Structural switch]]
-[[Category: Transferase]]