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[[Image:1akd.gif|left|200px]]<br />
<applet load="1akd" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1akd, resolution 1.8&Aring;" />
'''CYTOCHROME P450CAM FROM PSEUDOMONAS PUTIDA, COMPLEXED WITH 1S-CAMPHOR'''<br />


==Overview==
==CYTOCHROME P450CAM FROM PSEUDOMONAS PUTIDA, COMPLEXED WITH 1S-CAMPHOR==
The crystal structure of cytochrome P-450cam complexed with the enantiomer, (1S)-camphor has been solved to 1.8 angstroms resolution and compared with, the structure of the (1R)-camphor P-450cam complex. The overall protein, structure is the same for both enantiomer complexes. However, the, orientation of the substrates in the heme pocket differs. In contrast to, (1R)-camphor, the (1S)-enantiomer binds in at least two orientations. The, major binding mode of (1S)-camphor resembles the one of the, (1R)-enantiomer in that there is a hydrogen bond between Tyr-96 and the, quinone group of camphor, and the 10-methyl group points towards the, I-helix. The binding differs in that C-5 is not at a position suitable for, hydroxylation. In the other orientation (1S)-camphor is not hydrogen, bonded, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9357977 (full description)]]
<StructureSection load='1akd' size='340' side='right'caption='[[1akd]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1akd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AKD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AKD FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CAM:CAMPHOR'>CAM</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1akd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1akd OCA], [https://pdbe.org/1akd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1akd RCSB], [https://www.ebi.ac.uk/pdbsum/1akd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1akd ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CPXA_PSEPU CPXA_PSEPU] Involved in a camphor oxidation system.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ak/1akd_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1akd ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1AKD is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]] with K, HEM and CAM as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Camphor_5-monooxygenase Camphor 5-monooxygenase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.15.1 1.14.15.1]]. Structure known Active Sites: HEM and K. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AKD OCA]].
*[[Cytochrome P450 3D structures|Cytochrome P450 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Crystal structure of cytochrome P-450cam complexed with the (1S)-camphor enantiomer., Schlichting I, Jung C, Schulze H, FEBS Lett. 1997 Oct 6;415(3):253-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9357977 9357977]
[[Category: Large Structures]]
[[Category: Camphor 5-monooxygenase]]
[[Category: Pseudomonas putida]]
[[Category: Pseudomonas putida]]
[[Category: Single protein]]
[[Category: Jung C]]
[[Category: Jung, C.]]
[[Category: Schlichting I]]
[[Category: Schlichting, I.]]
[[Category: Schulze H]]
[[Category: Schulze, H.]]
[[Category: CAM]]
[[Category: HEM]]
[[Category: K]]
[[Category: cytochrome p450]]
[[Category: electron transport]]
[[Category: monooxygenase]]
[[Category: oxidoreductase]]
[[Category: oxygenase]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:33:33 2007''

Latest revision as of 09:31, 7 February 2024

CYTOCHROME P450CAM FROM PSEUDOMONAS PUTIDA, COMPLEXED WITH 1S-CAMPHORCYTOCHROME P450CAM FROM PSEUDOMONAS PUTIDA, COMPLEXED WITH 1S-CAMPHOR

Structural highlights

1akd is a 1 chain structure with sequence from Pseudomonas putida. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.8Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CPXA_PSEPU Involved in a camphor oxidation system.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1akd, resolution 1.80Å

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