4a45: Difference between revisions
New page: '''Unreleased structure''' The entry 4a45 is ON HOLD until Paper Publication Authors: Ficko-Blean, E., Stuart, C.P., Suits, M.D., Cid, M., Tessier, M., Woods, R.J., Boraston, A.B. Desc... |
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==CpGH89CBM32-5, from Clostridium perfringens, in complex with GalNAc- beta-1,3-galactose== | |||
<StructureSection load='4a45' size='340' side='right'caption='[[4a45]], [[Resolution|resolution]] 1.75Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4a45]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A45 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4A45 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4a45 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a45 OCA], [https://pdbe.org/4a45 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4a45 RCSB], [https://www.ebi.ac.uk/pdbsum/4a45 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4a45 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q8XM24_CLOPE Q8XM24_CLOPE] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
CpGH89 is a large multimodular enzyme produced by the human and animal pathogen Clostridium perfringens. The catalytic activity of this exo-alpha-d-N-acetylglucosaminidase is directed towards a rare carbohydrate motif, N-acetyl-beta-d-glucosamine-alpha-1,4-d-galactose, which is displayed on the class III mucins deep within the gastric mucosa. In addition to the family 89 glycoside hydrolase catalytic module this enzyme has six modules that share sequence similarity to the family 32 carbohydrate-binding modules (CBM32s), suggesting the enzyme has considerable capacity to adhere to carbohydrates. Here we suggest that two of the modules, CBM32-1 and CBM32-6, are not functional as carbohydrate-binding modules (CBMs) and demonstrate that three of the CBMs, CBM32-3, CBM32-4, and CBM32-5, are indeed capable of binding carbohydrates. CBM32-3 and CBM32-4 have a novel binding specificity for N-acetyl-beta-d-glucosamine-alpha-1,4-d-galactose, which thus complements the specificity of the catalytic module. The X-ray crystal structure of CBM32-4 in complex with this disaccharide reveals a mode of recognition that is based primarily on accommodation of the unique bent shape of this sugar. In contrast, as revealed by a series of X-ray crystal structures and quantitative binding studies, CBM32-5 displays the structural and functional features of galactose binding that is commonly associated with CBM family 32. The functional CBM32s that CpGH89 contains suggest the possibility for multivalent binding events and the partitioning of this enzyme to highly specific regions within the gastrointestinal tract. | |||
Carbohydrate Recognition by an Architecturally Complex alpha-N-Acetylglucosaminidase from Clostridium perfringens.,Ficko-Blean E, Stuart CP, Suits MD, Cid M, Tessier M, Woods RJ, Boraston AB PLoS One. 2012;7(3):e33524. Epub 2012 Mar 27. PMID:22479408<ref>PMID:22479408</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4a45" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Clostridium perfringens]] | |||
[[Category: Large Structures]] | |||
[[Category: Boraston AB]] | |||
[[Category: Cid M]] | |||
[[Category: Ficko-Blean E]] | |||
[[Category: Stuart CP]] | |||
[[Category: Suits MD]] | |||
[[Category: Tessier M]] | |||
[[Category: Woods RJ]] | |||