3bh0: Difference between revisions

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-[[Image:3bh0.jpg|left|200px]]<br /><applet load="3bh0" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="3bh0, resolution 2.35&Aring;" />
-'''ATPase Domain of G40P'''<br />
-==Overview==
+==ATPase Domain of G40P==
-Helicases are essential enzymes for DNA replication, a fundamental process, in all living organisms. The DnaB family are hexameric replicative, helicases that unwind duplex DNA and coordinate with RNA primase and other, proteins at the replication fork in prokaryotes. Here, we report the, full-length crystal structure of G40P, a DnaB family helicase. The hexamer, complex reveals an unusual architectural feature and a new type of, assembly mechanism. The hexamer has two tiers: a three-fold symmetric, N-terminal tier and a six-fold symmetric C-terminal tier. Monomers with, two different conformations, termed cis and trans, come together to, provide a topological solution for the dual symmetry within a hexamer., Structure-guided mutational studies indicate an important role for the, N-terminal tier in binding primase and regulating primase-mediated, stimulation of helicase activity. This study provides insights into the, structural and functional interplay between G40P helicase and DnaG, primase.
+<StructureSection load='3bh0' size='340' side='right'caption='[[3bh0]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3bh0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_phage_SPP1 Bacillus phage SPP1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BH0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BH0 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bh0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bh0 OCA], [https://pdbe.org/3bh0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bh0 RCSB], [https://www.ebi.ac.uk/pdbsum/3bh0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bh0 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q38152_BPSPP Q38152_BPSPP]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bh/3bh0_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bh0 ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-BH0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_sp6 Enterobacteria phage sp6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BH0 OCA].
+*[[Helicase 3D structures|Helicase 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-The structure of a DnaB-family replicative helicase and its interactions with primase., Wang G, Klein MG, Tokonzaba E, Zhang Y, Holden LG, Chen XS, Nat Struct Mol Biol. 2008 Jan;15(1):94-100. Epub 2007 Dec 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18157148 18157148]
+[[Category: Bacillus phage SPP1]]
-[[Category: Enterobacteria phage sp6]]
+[[Category: Large Structures]]
-[[Category: Single protein]]
+[[Category: Chen XS]]
-[[Category: Chen, X.S.]]
+[[Category: Holden LG]]
-[[Category: Holden, L.G.]]
+[[Category: Klein MG]]
-[[Category: Klein, M.G.]]
+[[Category: Tokonzaba E]]
-[[Category: Tokonzaba, E.]]
+[[Category: Wang G]]
-[[Category: Wang, G.]]
+[[Category: Zhang Y]]
-[[Category: Zhang, Y.]]
-[[Category: atpase]]
-[[Category: helicase]]
-[[Category: replication]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jan 31 11:02:34 2008''