3zsf: Difference between revisions
New page: '''Unreleased structure''' The entry 3zsf is ON HOLD until Paper Publication Authors: Bulut, H., Moniot, S., Scheffel, F., Gathmann, S., Licht, A., Saenger, W., Schneider, E. Descripti... |
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==Crystal structure of the L-cystine solute receptor of Neisseria gonorrhoeae in the unliganded open conformation== | |||
<StructureSection load='3zsf' size='340' side='right'caption='[[3zsf]], [[Resolution|resolution]] 2.32Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3zsf]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_gonorrhoeae Neisseria gonorrhoeae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZSF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZSF FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.32Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zsf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zsf OCA], [https://pdbe.org/3zsf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zsf RCSB], [https://www.ebi.ac.uk/pdbsum/3zsf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zsf ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q5F9M1_NEIG1 Q5F9M1_NEIG1] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
ATP-binding cassette (ABC) transporters are integral membrane proteins that carry a variety of substrates across biological membranes at the expense of ATP. The here considered prokaryotic canonical importers consist of three entities: an extracellular solute receptor, two membrane-intrinsic proteins forming a translocation pathway, and two cytoplasmic ATP-binding subunits. The ngo0372-74 and ngo2011-14 gene clusters from the human pathogen Neisseria gonorrhoeae were predicted by sequence homology as ABC transporters for the uptake of cystine and cysteine, respectively, and chosen for structural characterization. The structure of the receptor component Ngo0372 was obtained in a ligand-free "open" conformation and in a "closed" conformation when co-crystallized with l-cystine. Our data provide the first structural information of an l-cystine ABC transporter. Dissociation constants of 21 and 33 nM for l-cystine and l-selenocystine, respectively, were determined by isothermal titration calorimetry. In contrast, l-cystathionine and l-djenkolic acid are weak binders, while no binding was detectable for S-methyl-l-cysteine. Mutational analysis of two residues from the binding pocket, Trp97 and Tyr59, revealed that the latter is crucial for l-cystine binding. The structure of the Ngo2014 receptor was obtained in closed conformation in complex with co-purified l-cysteine. The protein binds l-cysteine with a K(d) of 26 nM. Comparison of the structures of both receptors and analysis of the ligand binding sites shed light on the mode of ligand recognition and provides insight into the tight binding of both substrates. Moreover, since l-cystine limitation leads to reduction in virulence of N. gonorrhoeae, Ngo0372 might be suited as target for an antimicrobial vaccine. | |||
Crystal Structures of Two Solute Receptors for l-Cystine and l-Cysteine, Respectively, of the Human Pathogen Neisseria gonorrhoeae.,Bulut H, Moniot S, Licht A, Scheffel F, Gathmann S, Saenger W, Schneider E J Mol Biol. 2012 Jan 20;415(3):560-72. Epub 2011 Nov 23. PMID:22138345<ref>PMID:22138345</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3zsf" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Neisseria gonorrhoeae]] | |||
[[Category: Bulut H]] | |||
[[Category: Gathmann S]] | |||
[[Category: Licht A]] | |||
[[Category: Moniot S]] | |||
[[Category: Saenger W]] | |||
[[Category: Scheffel F]] | |||
[[Category: Schneider E]] | |||