2ezs: Difference between revisions

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OCA

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-[[Image:2ezs.gif|left|200px]]<br /><applet load="2ezs" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="2ezs" />
-'''SOLUTION NMR STRUCTURE OF ECTODOMAIN OF SIV GP41, MODELS 31-40 OF AN ENSEMBLE OF 40 SIMULATED ANNEALING STRUCTURES'''<br />
-==Overview==
+==SOLUTION NMR STRUCTURE OF ECTODOMAIN OF SIV GP41, MODELS 31-40 OF AN ENSEMBLE OF 40 SIMULATED ANNEALING STRUCTURES==
-The solution structure of the ectodomain of simian immunodeficiency virus, (SIV) gp41 (e-gp41), consisting of residues 27-149, has been determined by, multidimensional heteronuclear NMR spectroscopy. SIV e-gp41 is a symmetric, 44 kDa trimer with each subunit consisting of antiparallel N-terminal, (residues 30-80) and C-terminal (residues 107-147) helices connected by a, 26 residue loop (residues 81-106). The N-terminal helices of each subunit, form a parallel coiled-coil structure in the interior of the complex which, is surrounded by the C-terminal helices located on the exterior of the, complex. The loop region is ordered and displays numerous intermolecular, and non-sequential intramolecular contacts. The helical core of SIV e-gp41, is similar to recent X-ray structures of truncated constructs of the, helical core of HIV-1 e-gp41. The present structure establishes, unambiguously the connectivity of the N- and C-terminal helices in the, trimer, and characterizes the conformation of the intervening loop, which, has been implicated by mutagenesis and antibody epitope mapping to play a, key role in gp120 association. In conjunction with previous studies, the, solution structure of the SIV e-gp41 ectodomain provides insight into the, binding site of gp120 and the mechanism of cell fusion. The present, structure of SIV e-gp41 represents one of the largest protein structures, determined by NMR to date.
+<StructureSection load='2ezs' size='340' side='right'caption='[[2ezs]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2ezs]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Simian_immunodeficiency_virus Simian immunodeficiency virus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EZS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EZS FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ezs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ezs OCA], [https://pdbe.org/2ezs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ezs RCSB], [https://www.ebi.ac.uk/pdbsum/2ezs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ezs ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q88031_SIV Q88031_SIV]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ez/2ezs_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ezs ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-EZS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Simian_immunodeficiency_virus Simian immunodeficiency virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EZS OCA].
+*[[Gp41 3D Structures|Gp41 3D Structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Three-dimensional solution structure of the 44 kDa ectodomain of SIV gp41., Caffrey M, Cai M, Kaufman J, Stahl SJ, Wingfield PT, Covell DG, Gronenborn AM, Clore GM, EMBO J. 1998 Aug 17;17(16):4572-84. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9707417 9707417]
+[[Category: Large Structures]]
 [[Category: Simian immunodeficiency virus]]
-[[Category: Single protein]]
+[[Category: Caffrey M]]
-[[Category: Caffrey, M.]]
+[[Category: Clore GM]]
-[[Category: Clore, G.M.]]
+[[Category: Gronenborn AM]]
-[[Category: Gronenborn, A.M.]]
-[[Category: siv gp41 ectodomain]]
-[[Category: virus envelope protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 19:25:01 2008''