2bm6: Difference between revisions

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-[[Image:2bm6.gif|left|200px]]<br />
-<applet load="2bm6" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="2bm6, resolution 2.2&Aring;" />
-'''THE STRUCTURE OF MFPA (RV3361C, C2221 CRYSTAL FORM). THE PENTAPEPTIDE REPEAT PROTEIN FROM MYCOBACTERIUM TUBERCULOSIS FOLDS AS A RIGHT-HANDED QUADRILATERAL BETA-HELIX.'''<br />
-==Overview==
+==The Structure of MfpA (Rv3361c, C2221 Crystal form). The Pentapeptide Repeat Protein from Mycobacterium tuberculosis Folds as A Right- handed Quadrilateral Beta-helix.==
-Fluoroquinolones are gaining increasing importance in the treatment of, tuberculosis. The expression of MfpA, a member of the pentapeptide repeat, family of proteins from Mycobacterium tuberculosis, causes resistance to, ciprofloxacin and sparfloxacin. This protein binds to DNA gyrase and, inhibits its activity. Its three-dimensional structure reveals a fold, which we have named the right-handed quadrilateral beta helix, that, exhibits size, shape, and electrostatic similarity to B-form DNA. This, represents a form of DNA mimicry and explains both its inhibitory effect, on DNA gyrase and fluoroquinolone resistance resulting from the protein's, expression in vivo.
+<StructureSection load='2bm6' size='340' side='right'caption='[[2bm6]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[2bm6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BM6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BM6 FirstGlance]. <br>
-BM6 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]] with CS as [[http://en.wikipedia.org/wiki/ligand ligand]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BM6 OCA]].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CS:CESIUM+ION'>CS</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bm6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bm6 OCA], [https://pdbe.org/2bm6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bm6 RCSB], [https://www.ebi.ac.uk/pdbsum/2bm6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bm6 ProSAT]</span></td></tr>
-A fluoroquinolone resistance protein from Mycobacterium tuberculosis that mimics DNA., Hegde SS, Vetting MW, Roderick SL, Mitchenall LA, Maxwell A, Takiff HE, Blanchard JS, Science. 2005 Jun 3;308(5727):1480-3. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15933203 15933203]
+</table>
-[[Category: Mycobacterium tuberculosis]]
+== Function ==
-[[Category: Single protein]]
+[https://www.uniprot.org/uniprot/O50390_MYCTO O50390_MYCTO]
-[[Category: Blanchard, J.S.]]
+== Evolutionary Conservation ==
-[[Category: Hegde, S.S.]]
+[[Image:Consurf_key_small.gif|200px|right]]
-[[Category: Maxwell, A.]]
+Check<jmol>
-[[Category: Mitchenall, L.A.]]
+  <jmolCheckbox>
-[[Category: Roderick, S.L.]]
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bm/2bm6_consurf.spt"</scriptWhenChecked>
-[[Category: Takiff, H.E.]]
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-[[Category: Vetting, M.W.]]
+    <text>to colour the structure by Evolutionary Conservation</text>
-[[Category: CS]]
+  </jmolCheckbox>
-[[Category: dna gyrase]]
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bm6 ConSurf].
-[[Category: dna mimicry]]
+<div style="clear:both"></div>
-[[Category: fluroquinolone resistance]]
+__TOC__
-[[Category: pentapeptide repeat protein]]
+</StructureSection>
-[[Category: right-handed quadrilateral beta-helix]]
+[[Category: Large Structures]]
+[[Category: Mycobacterium tuberculosis H37Rv]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:05:54 2007''
+[[Category: Blanchard JS]]
+[[Category: Hegde SS]]
+[[Category: Maxwell A]]
+[[Category: Mitchenall LA]]
+[[Category: Roderick SL]]
+[[Category: Takiff HE]]
+[[Category: Vetting MW]]