2co2: Difference between revisions

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New page: left|200px<br /><applet load="2co2" size="350" color="white" frame="true" align="right" spinBox="true" caption="2co2, resolution 2.30Å" /> '''SALMONELLA ENTERICA ...
 
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[[Image:2co2.gif|left|200px]]<br /><applet load="2co2" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2co2, resolution 2.30&Aring;" />
'''SALMONELLA ENTERICA SAFA PILIN IN COMPLEX WITH A 19-RESIDUE SAFA NTE PEPTIDE (F3A MUTANT)'''<br />


==Overview==
==Salmonella enterica SafA pilin in complex with a 19-residue SafA Nte peptide (F3A mutant)==
Gram-negative pathogens commonly use the chaperone-usher pathway to, assemble adhesive multisubunit fibers on their surface. In the periplasm, subunits are stabilized by a chaperone that donates a beta strand to, complement the subunits' truncated immunoglobulin-like fold. Pilus, assembly proceeds through a "donor-strand exchange" (DSE) mechanism, whereby this complementary beta strand is replaced by the N-terminal, extension (Nte) of an incoming pilus subunit. Using X-ray crystallography, and real-time electrospray ionization mass spectrometry (ESI-MS), we, demonstrate that DSE requires the formation of a transient ternary complex, between the chaperone-subunit complex and the Nte of the next subunit to, be assembled. The process is crucially dependent on an initiation site, (the P5 pocket) needed to recruit the incoming Nte. The data also suggest, a capping reaction displacing DSE toward product formation. These results, support a zip-in-zip-out mechanism for DSE and a catalytic role for the, usher, the molecular platform at which pili are assembled.
<StructureSection load='2co2' size='340' side='right'caption='[[2co2]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2co2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica Salmonella enterica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CO2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CO2 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2co2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2co2 OCA], [https://pdbe.org/2co2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2co2 RCSB], [https://www.ebi.ac.uk/pdbsum/2co2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2co2 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q8ZRK4_SALTY Q8ZRK4_SALTY]


==About this Structure==
==See Also==
2CO2 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Salmonella_enterica Salmonella enterica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CO2 OCA].
*[[Pilin 3D structures|Pilin 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Donor-strand exchange in chaperone-assisted pilus assembly proceeds through a concerted beta strand displacement mechanism., Remaut H, Rose RJ, Hannan TJ, Hultgren SJ, Radford SE, Ashcroft AE, Waksman G, Mol Cell. 2006 Jun 23;22(6):831-42. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16793551 16793551]
[[Category: Large Structures]]
[[Category: Protein complex]]
[[Category: Salmonella enterica]]
[[Category: Salmonella enterica]]
[[Category: Ashcroft, A.E.]]
[[Category: Ashcroft AE]]
[[Category: Hannan, T.J.]]
[[Category: Hannan TJ]]
[[Category: Hultgren, S.J.]]
[[Category: Hultgren SJ]]
[[Category: Radford, S.E.]]
[[Category: Radford SE]]
[[Category: Remaut, H.]]
[[Category: Remaut H]]
[[Category: Rose, R.J.]]
[[Category: Rose RJ]]
[[Category: Waksman, G.]]
[[Category: Waksman G]]
[[Category: fibril protein]]
[[Category: fold complementation]]
[[Category: pilus subunit]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 18:45:23 2008''

Latest revision as of 09:39, 1 May 2024

Salmonella enterica SafA pilin in complex with a 19-residue SafA Nte peptide (F3A mutant)Salmonella enterica SafA pilin in complex with a 19-residue SafA Nte peptide (F3A mutant)

Structural highlights

2co2 is a 2 chain structure with sequence from Salmonella enterica. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.3Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q8ZRK4_SALTY

See Also

2co2, resolution 2.30Å

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