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New page: left|200px<br /><applet load="2afv" size="350" color="white" frame="true" align="right" spinBox="true" caption="2afv, resolution 3.00Å" /> '''The Crystal Structur...
 
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[[Image:2afv.gif|left|200px]]<br /><applet load="2afv" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2afv, resolution 3.00&Aring;" />
'''The Crystal Structure of Putative Precorrin Isomerase CbiC in Cobalamin Biosynthesis'''<br />


==Overview==
==The Crystal Structure of Putative Precorrin Isomerase CbiC in Cobalamin Biosynthesis==
The leptospira cbiC encodes the enzyme catalyzing the methyl rearrangement, reaction of the cobalamin biosynthesis pathway. The protein has been, cloned and overexpressed as a His-tagged recombinant protein in, Escherichia coli. The crystal structures have been solved in two crystal, forms (P4(2)2(1)2 and P3(1)21) diffracting to 3.0 and 2.3A resolution, respectively. The structures are similar to the precorrin-8x methyl mutase, (CobH), an enzyme of the aerobic pathway to vitamin B12.
<StructureSection load='2afv' size='340' side='right'caption='[[2afv]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2afv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Leptospira_interrogans Leptospira interrogans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AFV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AFV FirstGlance]. <br>
2AFV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Leptospira_interrogans Leptospira interrogans] with <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=P6G:'>P6G</scene> and <scene name='pdbligand=P33:'>P33</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Precorrin-8X_methylmutase Precorrin-8X methylmutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.1.2 5.4.1.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AFV OCA].
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=P33:3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL'>P33</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2afv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2afv OCA], [https://pdbe.org/2afv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2afv RCSB], [https://www.ebi.ac.uk/pdbsum/2afv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2afv ProSAT]</span></td></tr>
The crystal structure of putative precorrin isomerase CbiC in cobalamin biosynthesis., Xue Y, Wei Z, Li X, Gong W, J Struct Biol. 2006 Mar;153(3):307-11. Epub 2005 Dec 27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16427313 16427313]
</table>
== Function ==
[https://www.uniprot.org/uniprot/CBIC_LEPIN CBIC_LEPIN] Catalyzes the conversion of cobalt-precorrin-8 to cobyrinate.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/af/2afv_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2afv ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Leptospira interrogans]]
[[Category: Leptospira interrogans]]
[[Category: Precorrin-8X methylmutase]]
[[Category: Wei Z]]
[[Category: Single protein]]
[[Category: Xue Y]]
[[Category: Wei, Z.]]
[[Category: Xue, Y.]]
[[Category: CL]]
[[Category: P33]]
[[Category: P6G]]
[[Category: isomerase]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 18:01:18 2008''

Latest revision as of 16:41, 13 March 2024

The Crystal Structure of Putative Precorrin Isomerase CbiC in Cobalamin BiosynthesisThe Crystal Structure of Putative Precorrin Isomerase CbiC in Cobalamin Biosynthesis

Structural highlights

2afv is a 2 chain structure with sequence from Leptospira interrogans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CBIC_LEPIN Catalyzes the conversion of cobalt-precorrin-8 to cobyrinate.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

2afv, resolution 3.00Å

Drag the structure with the mouse to rotate

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