2e0z: Difference between revisions

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New page: left|200px<br /><applet load="2e0z" size="350" color="white" frame="true" align="right" spinBox="true" caption="2e0z, resolution 3.60Å" /> '''Crystal structure of...
 
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[[Image:2e0z.gif|left|200px]]<br /><applet load="2e0z" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2e0z, resolution 3.60&Aring;" />
'''Crystal structure of virus-like particle from Pyrococcus furiosus'''<br />


==Overview==
==Crystal structure of virus-like particle from Pyrococcus furiosus==
Pyrococcus furiosus is a hyperthermophilic archaeal microorganism found, near deep-sea thermal vents and its optimal growth temperature of 100, degrees C. Recently, a 38.8-kDa protein from P. furiosus DSM 3638 was, isolated and characterized. Electron microscopy revealed that this protein, aggregated as spheres of approximately 30 nm in diameter, which we, designated P. furiosus virus-like particles (PfVs). X-ray crystallographic, analysis at 3.6-A resolution revealed that each PfV consisted of 180, copies of the 38.8-kDa protein and retained T=3 icosahedral symmetry, as, is often the case in spherical viruses. The total molecular mass of each, particle was approximately 7 MDa. An examination of capsid structures, suggested strong evolutionary links among PfV, tailed double-stranded DNA, bacteriophages, and herpes viruses. The similar three-dimensional, structures of the various coat proteins indicate that these viral capsids, might have originated and evolved from a common ancestor. The structure of, PfV provides a previously undescribed example of viral relationships, across the three domains of life (Eukarya, Bacteria, and Archaea).
<StructureSection load='2e0z' size='340' side='right'caption='[[2e0z]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2e0z]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E0Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2E0Z FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.6&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2e0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2e0z OCA], [https://pdbe.org/2e0z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2e0z RCSB], [https://www.ebi.ac.uk/pdbsum/2e0z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2e0z ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ENCP1_PYRFU ENCP1_PYRFU] Fusion of the shell and cargo protein of a type 1 encapsulin nanocompartment (PubMed:17397865). The nanocompartment is probably involved in iron storage (Probable). Expression in E.coli generates spherical particles (PfSPs) about 30 nm in diameter (PubMed:16091594).<ref>PMID:16091594</ref> <ref>PMID:17397865</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e0/2e0z_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2e0z ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2E0Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E0Z OCA].
*[[Art:Faberge Eggs|Art:Faberge Eggs]]
 
== References ==
==Reference==
<references/>
The Crystal Structure of a Virus-like Particle from the Hyperthermophilic Archaeon Pyrococcus furiosus Provides Insight into the Evolution of Viruses., Akita F, Chong KT, Tanaka H, Yamashita E, Miyazaki N, Nakaishi Y, Suzuki M, Namba K, Ono Y, Tsukihara T, Nakagawa A, J Mol Biol. 2007 Mar 2;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17397865 17397865]
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pyrococcus furiosus]]
[[Category: Pyrococcus furiosus]]
[[Category: Single protein]]
[[Category: Akita F]]
[[Category: Akita, F.]]
[[Category: Chong KT]]
[[Category: Chong, K.T.]]
[[Category: Miyazaki N]]
[[Category: Miyazaki, N.]]
[[Category: Nakagawa A]]
[[Category: Nakagawa, A.]]
[[Category: Nakaishi Y]]
[[Category: Nakaishi, Y.]]
[[Category: Namba K]]
[[Category: Namba, K.]]
[[Category: Ono Y]]
[[Category: Ono, Y.]]
[[Category: Suzuki M]]
[[Category: Suzuki, M.]]
[[Category: Tanaka H]]
[[Category: Tanaka, H.]]
[[Category: Tsukihara T]]
[[Category: Tsukihara, T.]]
[[Category: Yamashita E]]
[[Category: Yamashita, E.]]
[[Category: bacteriophage]]
[[Category: hk97]]
[[Category: t4]]
[[Category: virus]]
[[Category: virus-like particle]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:14:35 2008''

Latest revision as of 16:48, 13 March 2024

Crystal structure of virus-like particle from Pyrococcus furiosusCrystal structure of virus-like particle from Pyrococcus furiosus

Structural highlights

2e0z is a 3 chain structure with sequence from Pyrococcus furiosus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.6Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ENCP1_PYRFU Fusion of the shell and cargo protein of a type 1 encapsulin nanocompartment (PubMed:17397865). The nanocompartment is probably involved in iron storage (Probable). Expression in E.coli generates spherical particles (PfSPs) about 30 nm in diameter (PubMed:16091594).[1] [2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Namba K, Hagiwara K, Tanaka H, Nakaishi Y, Chong KT, Yamashita E, Armah GE, Ono Y, Ishino Y, Omura T, Tsukihara T, Nakagawa A. Expression and molecular characterization of spherical particles derived from the genome of the hyperthermophilic euryarchaeote Pyrococcus furiosus. J Biochem. 2005 Aug;138(2):193-9. PMID:16091594 doi:10.1093/jb/mvi111
  2. ↑ Akita F, Chong KT, Tanaka H, Yamashita E, Miyazaki N, Nakaishi Y, Suzuki M, Namba K, Ono Y, Tsukihara T, Nakagawa A. The crystal structure of a virus-like particle from the hyperthermophilic archaeon Pyrococcus furiosus provides insight into the evolution of viruses. J Mol Biol. 2007 May 18;368(5):1469-83. Epub 2007 Mar 2. PMID:17397865 doi:10.1016/j.jmb.2007.02.075

2e0z, resolution 3.60Å

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