Sandbox Reserved 327: Difference between revisions
| (2 intermediate revisions by the same user not shown) | |||
| Line 26: | Line 26: | ||
''In vitro'' mutation in the eIF1-NTT area would alter the binding of eIF5 and eIF2β, but not eIF3c which supported that this area played role in stimulating the MFC assembly <ref name="eIF1"/>. ''In vivo'' study also supported that eIF1-NTT would allow the binding of eIF1 to the eIF5 and eIF2β <ref name="eIF1"/>. | ''In vitro'' mutation in the eIF1-NTT area would alter the binding of eIF5 and eIF2β, but not eIF3c which supported that this area played role in stimulating the MFC assembly <ref name="eIF1"/>. ''In vivo'' study also supported that eIF1-NTT would allow the binding of eIF1 to the eIF5 and eIF2β <ref name="eIF1"/>. | ||
===eIF1-KH=== | ===eIF1-KH=== | ||
The other binding site area of eIF1 to eIF5 which is believed to play role in the AUG start codon selection since ''in vitro'' mutation in this area by altering the basic | The other binding site area of eIF1 to eIF5 which is believed to play role in the AUG start codon selection since ''in vitro'' mutation in this area by altering the basic part of the KH region would relax the start codon selection <ref name="eIF1"/>.The mutation would also alter the formation of MFC, but not like mutation in the eIF1-NTT area, eIF1-NTT mutation is lethal to the yeast growth <ref name="eIF1"/>. Mutation that alter the hydrophobic residue would disrupt a critical link to the PIC and prevent the eIF1 dissociation <ref name="cheung"/>. The critical link is believed to be strongly associate with eIF5 <ref name="eIF1"/>. | ||
=Function and Mechanism= | =Function and Mechanism= | ||
[[Image:640px-Eukaryotic_initiation.png|thumb|left| | [[Image:640px-Eukaryotic_initiation.png|thumb|left|570px|Simplified model of eukaryotic initiation by [http://commons.wikimedia.org/wiki/User:Webridge Webridge]]] | ||
eIF1 is essential to control the ribosome conformational rearrangement in translation initiation by stimulating the formation of MFC and controlling the start codon selection which allow the translation elongation process to occur | eIF1 is essential to control the ribosome conformational rearrangement in translation initiation by stimulating the formation of MFC and controlling the start codon selection which allow the translation elongation process to occur | ||