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[[Image:2idv.jpg|left|200px]]<br /><applet load="2idv" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2idv, resolution 2.300&Aring;" />
'''Crystal structure of wheat C113S mutant EIF4E bound TO 7-methyl-GDP'''<br />


==Overview==
==Crystal structure of wheat C113S mutant EIF4E bound TO 7-methyl-GDP==
Eukaryotic translation initiation factor-4E (eIF4E) recognizes and binds, the m(7) guanosine nucleotide at the 5' end of eukaryotic messenger RNAs;, this protein-RNA interaction is an essential step in the initiation of, protein synthesis. The structure of eIF4E from wheat (Triticum aestivum), was investigated using a combination of x-ray crystallography and nuclear, magnetic resonance (NMR) methods. The overall fold of the crystallized, protein was similar to eIF4E from other species, with eight beta-strands, three alpha-helices, and three extended loops. Surprisingly, the wild-type, protein did not crystallize with m(7)GTP in its binding site, despite the, ligand being present in solution; conformational changes in the, cap-binding loops created a large cavity at the usual cap-binding site., The eIF4E crystallized in a dimeric form with one of the cap-binding loops, of one monomer inserted into the cavity of the other. The protein also, contained an intramolecular disulfide bridge between two cysteines (Cys), that are conserved only in plants. A Cys-to-serine mutant of wheat eIF4E, which lacked the ability to form the disulfide, crystallized with m(7)GDP, in its binding pocket, with a structure similar to that of the eIF4E-cap, complex of other species. NMR spectroscopy was used to show that the Cys, that form the disulfide in the crystal are reduced in solution but can be, induced to form the disulfide under oxidizing conditions. The observation, that the disulfide-forming Cys are conserved in plants raises the, possibility that their oxidation state may have a role in regulating, protein function. NMR provided evidence that in oxidized eIF4E, the loop, that is open in the ligand-free crystal dimer is relatively flexible in, solution. An NMR-based binding assay showed that the reduced wheat eIF4E, the oxidized form with the disulfide, and the Cys-to-serine mutant protein, each bind m(7)GTP in a similar and labile manner, with dissociation rates, in the range of 20 to 100 s(-1).
<StructureSection load='2idv' size='340' side='right'caption='[[2idv]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2idv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IDV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IDV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=M7G:7N-METHYL-8-HYDROGUANOSINE-5-DIPHOSPHATE'>M7G</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2idv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2idv OCA], [https://pdbe.org/2idv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2idv RCSB], [https://www.ebi.ac.uk/pdbsum/2idv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2idv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/IF4E1_WHEAT IF4E1_WHEAT] Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/id/2idv_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2idv ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2IDV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum] with <scene name='pdbligand=M7G:'>M7G</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IDV OCA].
*[[Eukaryotic initiation factor 3D structures|Eukaryotic initiation factor 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
The structure of eukaryotic translation initiation factor-4E from wheat reveals a novel disulfide bond., Monzingo AF, Dhaliwal S, Dutt-Chaudhuri A, Lyon A, Sadow JH, Hoffman DW, Robertus JD, Browning KS, Plant Physiol. 2007 Apr;143(4):1504-18. Epub 2007 Feb 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17322339 17322339]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Triticum aestivum]]
[[Category: Triticum aestivum]]
[[Category: Browning, K.S.]]
[[Category: Browning KS]]
[[Category: Dhaliwal, S.]]
[[Category: Dhaliwal S]]
[[Category: Dutt-Chaudhuri, A.]]
[[Category: Dutt-Chaudhuri A]]
[[Category: Hoffman, D.W.]]
[[Category: Hoffman DW]]
[[Category: Monzingo, A.F.]]
[[Category: Monzingo AF]]
[[Category: Robertus, J.D.]]
[[Category: Robertus JD]]
[[Category: Sadow, J.]]
[[Category: Sadow J]]
[[Category: M7G]]
[[Category: cap]]
[[Category: eif4e]]
[[Category: eukaryotic initiation factor 4e]]
[[Category: methyl-7-gdp]]
[[Category: translation regulator]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:54:38 2008''

Latest revision as of 15:14, 6 March 2024

Crystal structure of wheat C113S mutant EIF4E bound TO 7-methyl-GDPCrystal structure of wheat C113S mutant EIF4E bound TO 7-methyl-GDP

Structural highlights

2idv is a 1 chain structure with sequence from Triticum aestivum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.3Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

IF4E1_WHEAT Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

2idv, resolution 2.30Å

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