2w0r: Difference between revisions

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-[[Image:2w0r.png|left|200px]]
-<!--
+==Crystal structure of the mutated N263D YscU C-terminal domain==
-The line below this paragraph, containing "STRUCTURE_2w0r", creates the "Structure Box" on the page.
+<StructureSection load='2w0r' size='340' side='right'caption='[[2w0r]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2w0r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Yersinia_enterocolitica Yersinia enterocolitica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W0R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W0R FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-{{STRUCTURE_2w0r|  PDB=2w0r  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w0r OCA], [https://pdbe.org/2w0r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w0r RCSB], [https://www.ebi.ac.uk/pdbsum/2w0r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w0r ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q56844_YEREN Q56844_YEREN]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w0/2w0r_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2w0r ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The inner-membrane protein YscU has an important role during the assembly of the Yersinia enterocolitica type III secretion injectisome. Its cytoplasmic domain (YscU(C)) recognizes translocators as individual substrates in the export hierarchy. Activation of YscU entails autocleavage at a conserved NPTH motif. Modification of this motif markedly changes the properties of YscU, including translocator export cessation and production of longer injectisome needles. We determined the crystal structures of the uncleaved variants N263A and N263D of YscU(C) at 2.05 A and 1.55 A resolution, respectively. The globular domain is found to consist of a central, mixed beta-sheet surrounded by alpha-helices. The NPTH motif forms a type II beta-turn connecting two beta-strands. NMR analysis of cleaved and uncleaved YscU(C) indicates that the global structure of the protein is retained in cleaved YscU(C). The structure of YscU(C) variant N263D reveals that wild type YscU(C) is poised for cleavage due to an optimal reaction geometry for nucleophilic attack of the scissile bond by the side chain of Asn263. In vivo analysis of N263Q and H266A/R314A YscU variants showed a phenotype that combines the absence of translocator secretion with normal needle-length control. Comparing the structure of YscU to those of related proteins reveals that the linker domain between the N-terminal transmembrane domain and the autocleavage domain can switch from an extended to a largely alpha-helical conformation, allowing for optimal positioning of the autocleavage domain during injectisome assembly.
-===CRYSTAL STRUCTURE OF THE MUTATED N263D YSCU C-TERMINAL DOMAIN===
+Structure of the type III secretion recognition protein YscU from Yersinia enterocolitica.,Wiesand U, Sorg I, Amstutz M, Wagner S, van den Heuvel J, Luhrs T, Cornelis GR, Heinz DW J Mol Biol. 2009 Jan 23;385(3):854-66. Epub 2008 Oct 19. PMID:18976663<ref>PMID:18976663</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_18976663}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 2w0r" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 18976663 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_18976663}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-[[2w0r]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Yersinia_enterocolitica Yersinia enterocolitica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W0R OCA].
-==Reference==
-<ref group="xtra">PMID:18976663</ref><ref group="xtra">PMID:8045883</ref><references group="xtra"/>
 [[Category: Yersinia enterocolitica]]
-[[Category: Amstutz, M.]]
+[[Category: Amstutz M]]
-[[Category: Cornelis, G R.]]
+[[Category: Cornelis GR]]
-[[Category: Heinz, D W.]]
+[[Category: Heinz DW]]
-[[Category: Heuvel, J Van Den.]]
+[[Category: Luehrs T]]
-[[Category: Luehrs, T.]]
+[[Category: Sorg I]]
-[[Category: Sorg, I.]]
+[[Category: Van Den Heuvel J]]
-[[Category: Wagner, S.]]
+[[Category: Wagner S]]
-[[Category: Wiesand, U.]]
+[[Category: Wiesand U]]
-[[Category: Autocleavage]]
-[[Category: Membrane protein]]
-[[Category: Plasmid]]
-[[Category: Recognition protein]]
-[[Category: Type iii secretion system]]