Colicin E3: Difference between revisions
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After binding to the BtuB receptor, the complex recruits OmpF, and a group of proteins from the [[Tol]] group of proteins, TolQRAB. The mechanism by which these proteins aid the translocation of ColE3 is as yet unknown, but the colicin then traverses the outer membrane and reaches the periplasm. | After binding to the BtuB receptor, the complex recruits OmpF, and a group of proteins from the [[Tol]] group of proteins, TolQRAB. The mechanism by which these proteins aid the translocation of ColE3 is as yet unknown, but the colicin then traverses the outer membrane and reaches the periplasm. | ||
For more information about the uptake of Colicin E3 see the uptake section on [[Colicin E9]], which has a similar mechanism. | |||
==Killing Activities== | ==Killing Activities== | ||
{{STRUCTURE_2xfz | PDB=2xfz | SCENE= Colicin_E3/Cole3crrna/1 }} | {{STRUCTURE_2xfz | PDB=2xfz | SCENE= Colicin_E3/Cole3crrna/1 }} | ||
The C terminal domain of colicin E3 kills the cells that it penetrates with its [[16s rRNase activity]], by actively degrading the 16s rRNA subunit of the cell's 70S ribosome. This prevents the cell from producing any proteins once attacked, which will lead to the death of the cell. This rRNA cleavage is specific ''in vivo'', but random when the rRNA domain is isolated ''in vitro''<ref> PMID: 368047 </ref>. The cleavage is unaffected by the presence of cellular ribonucleases<ref> PMID: 4567332 </ref>. The specific cleavage observed ''in vivo'' occurs within the ribosomal decoding A-site, in between A1493 and G1494, resulting in the complete halt of protein biosynthesis, leading to cell death<ref> PMID: 12423788 </ref>. | The C terminal domain of colicin E3 kills the cells that it penetrates with its [[16s rRNase activity]], by actively degrading the <scene name='Colicin_E3/16srrna/1'>16s rRNA</scene> subunit of the cell's 70S ribosome. This prevents the cell from producing any proteins once attacked, which will lead to the death of the cell. This rRNA cleavage is specific ''in vivo'', but random when the rRNA domain is isolated ''in vitro''<ref> PMID: 368047 </ref>. The cleavage is unaffected by the presence of cellular ribonucleases<ref> PMID: 4567332 </ref>. The specific cleavage observed ''in vivo'' occurs within the ribosomal decoding A-site, in between A1493 and G1494, resulting in the complete halt of protein biosynthesis, leading to cell death<ref> PMID: 12423788 </ref>. | ||
==References== | ==References== | ||
<references/> | <references/> | ||