1fi5: Difference between revisions

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-[[Image:1fi5.png|left|200px]]
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+==NMR STRUCTURE OF THE C TERMINAL DOMAIN OF CARDIAC TROPONIN C BOUND TO THE N TERMINAL DOMAIN OF CARDIAC TROPONIN I.==
-The line below this paragraph, containing "STRUCTURE_1fi5", creates the "Structure Box" on the page.
+<StructureSection load='1fi5' size='340' side='right'caption='[[1fi5]]' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1fi5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1ggs 1ggs]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FI5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FI5 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-{{STRUCTURE_1fi5|  PDB=1fi5  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fi5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fi5 OCA], [https://pdbe.org/1fi5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fi5 RCSB], [https://www.ebi.ac.uk/pdbsum/1fi5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fi5 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/TNNC1_CHICK TNNC1_CHICK] Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fi/1fi5_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fi5 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The N-terminal domain of cardiac troponin I (cTnI) comprising residues 33-80 and lacking the cardiac-specific amino terminus forms a stable binary complex with the C-terminal domain of cardiac troponin C (cTnC) comprising residues 81-161. We have utilized heteronuclear multidimensional NMR to assign the backbone and side-chain resonances of Ca2+-saturated cTnC(81-161) both free and bound to cTnI(33-80). No significant differences were observed between secondary structural elements determined for free and cTnI(33-80)-bound cTnC(81-161). We have determined solution structures of Ca2+-saturated cTnC(81-161) free and bound to cTnI(33-80). While the tertiary structure of cTnC(81-161) is qualitatively similar to that observed free in solution, the binding of cTnI(33-80) results mainly in an opening of the structure and movement of the loop region between helices F and G. Together, these movements provide the binding site for the N-terminal domain of cTnI. The putative binding site for cTnI(33-80) was determined by mapping amide proton and nitrogen chemical shift changes, induced by the binding of cTnI(33-80), onto the C-terminal cTnC structure. The binding interface for cTnI(33-80), as suggested from chemical shift changes, involves predominantly hydrophobic interactions located in the expanded hydrophobic pocket. The largest chemical shift changes were observed in the loop region connecting helices F and G. Inspection of available TnC sequences reveals that these residues are highly conserved, suggesting a common binding motif for the Ca2+/Mg2+-dependent interaction site in the TnC/TnI complex.
-===NMR STRUCTURE OF THE C TERMINAL DOMAIN OF CARDIAC TROPONIN C BOUND TO THE N TERMINAL DOMAIN OF CARDIAC TROPONIN I.===
+Solution structures of the C-terminal domain of cardiac troponin C free and bound to the N-terminal domain of cardiac troponin I.,Gasmi-Seabrook GM, Howarth JW, Finley N, Abusamhadneh E, Gaponenko V, Brito RM, Solaro RJ, Rosevear PR Biochemistry. 1999 Jun 29;38(26):8313-22. PMID:10387077<ref>PMID:10387077</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_10387077}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 1fi5" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 10387077 is the PubMed ID number.
--->
-{{ABSTRACT_PUBMED_10387077}}
-==About this Structure==
-[[1fi5]] is a 1 chain structure of [[Troponin]] with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1ggs 1ggs]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FI5 OCA].
 ==See Also==
-*[[Troponin]]
+*[[Troponin 3D structures|Troponin 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:10387077</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Gallus gallus]]
-[[Category: Abusamhadneh, E.]]
+[[Category: Large Structures]]
-[[Category: Brito, R M.]]
+[[Category: Abusamhadneh E]]
-[[Category: Finley, N.]]
+[[Category: Brito RM]]
-[[Category: Gaponenko, V.]]
+[[Category: Finley N]]
-[[Category: Gasmi-Seabrook, G M.]]
+[[Category: Gaponenko V]]
-[[Category: Howarth, J W.]]
+[[Category: Gasmi-Seabrook GM]]
-[[Category: Rosevear, P R.]]
+[[Category: Howarth JW]]
-[[Category: Solaro, R J.]]
+[[Category: Rosevear PR]]
-[[Category: Calcium binding protein]]
+[[Category: Solaro RJ]]
-[[Category: Cardiac]]
-[[Category: Contractile protein]]
-[[Category: Muscle protein]]
-[[Category: Troponin c-troponin i interaction]]