1kuq: Difference between revisions

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-[[Image:1kuq.png|left|200px]]
-<!--
+==CRYSTAL STRUCTURE OF T3C MUTANT S15 RIBOSOMAL PROTEIN IN COMPLEX WITH 16S RRNA==
-The line below this paragraph, containing "STRUCTURE_1kuq", creates the "Structure Box" on the page.
+<StructureSection load='1kuq' size='340' side='right'caption='[[1kuq]], [[Resolution|resolution]] 2.84&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1kuq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KUQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KUQ FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.84&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-{{STRUCTURE_1kuq|  PDB=1kuq  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kuq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kuq OCA], [https://pdbe.org/1kuq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kuq RCSB], [https://www.ebi.ac.uk/pdbsum/1kuq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kuq ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/RS15_THETH RS15_THETH] One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it helps nucleate assembly of the platform of the 30S subunit by binding and bridging several RNA helices of the 16S rRNA.  Forms an intersubunit bridge (bridge B4) with the 23S rRNA of the 50S subunit in the ribosome (By similarity).
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ku/1kuq_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kuq ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The position and conformation of the N-terminal helix of free ribosomal protein S15 was earlier found to be modified under various conditions. This variability was supposed to provide the recognition by the protein of its specific site on 16S rRNA. To test this hypothesis, we substituted some amino acid residues in this helix and assessed effects of these substitutions on the affinity of the protein for 16S rRNA. The crystal structure of the complex of one of these mutants (Thr3Cys S15) with the 16S rRNA fragment was determined, and a computer model of the complex containing another mutant (Gln8Met S15) was designed. The available and new information was analyzed in detail, and the N-terminal helix was concluded to play no significant role in the specific binding of the S15 protein to its target on 16S rRNA.
-===CRYSTAL STRUCTURE OF T3C MUTANT S15 RIBOSOMAL PROTEIN IN COMPLEX WITH 16S RRNA===
+Role of N-terminal helix in interaction of ribosomal protein S15 with 16S rRNA.,Revtovich SV, Nikulin AD, Nikonov SV Biochemistry (Mosc). 2004 Dec;69(12):1319-23. PMID:15627386<ref>PMID:15627386</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_15627386}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 1kuq" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 15627386 is the PubMed ID number.
--->
-{{ABSTRACT_PUBMED_15627386}}
-==About this Structure==
-[[1kuq]] is a 2 chain structure of [[Ribosomal protein S15]] with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KUQ OCA].
 ==See Also==
-*[[Ribosomal protein S15]]
+*[[Ribosomal protein S15|Ribosomal protein S15]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:15627386</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Thermus thermophilus]]
-[[Category: Dumas, P.]]
+[[Category: Dumas P]]
-[[Category: Ehresmann, B.]]
+[[Category: Ehresmann B]]
-[[Category: Ehresmann, C.]]
+[[Category: Ehresmann C]]
-[[Category: Garber, M.]]
+[[Category: Garber M]]
-[[Category: Nevskaya, N.]]
+[[Category: Nevskaya N]]
-[[Category: Nikonov, S.]]
+[[Category: Nikonov S]]
-[[Category: Nikulin, A D.]]
+[[Category: Nikulin AD]]
-[[Category: Revtovich, S.]]
+[[Category: Revtovich S]]
-[[Category: Tishchenko, S.]]
+[[Category: Tishchenko S]]
-[[Category: Ribosome]]
-[[Category: Rrna-protein complex]]