Proteasome: Difference between revisions

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Alexander Berchansky, Michal Harel, Joel L. Sussman, Jaime Prilusky

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-[[Image:3gpt.jpg|left|200px|thumb|Crystal Structure of Proteasome, [[3gpt]]]]
+<StructureSection load='' size='350' side='right' scene='41/417543/Cv/1' caption='Yeast proteasome 20S core complex with salinosporamide derivative, [[3gpt]]'>
-{{STRUCTURE_3gpt|  PDB=3gpt  | SIZE=300| SCENE= |right|CAPTION=Proteasome, [[3gpt]] }}
+== Function ==
+[[Proteasome]] (PRTS) are large protein complexes which degrade unneeded proteins into small polypeptides<ref>PMID:22350895</ref>.  The '''26S PRTS''' is composed of a central '''20S core particle''' which contains 4 stacked rings each with several members and two 19S caps.  The 19S cap is composed of a base with 10 proteins six of which are ATPases and a lid which contains 9 proteins which bind polyubiquitin. <br />
+*'''13S PRTS''' is a PRTS core intermediate containing α ring, partial β ring and 3 chaperones<ref>PMID:33846632</ref>.
+*'''15S PRTS''' is half of an 20S PRTS and upon dimerisation forms the mature 20S PRTS<ref>PMID:32442437</ref>.
+For more details see [[3unb]].
+== Structural highlights ==
-[[Proteasome]] (PRTS) are large protein complexes which degrade unneeded proteins into small polypeptides.  The 26S PRTS is composed of a central 20S core particle which contains 4 stacked rings each with several members and two 19S caps.  The core particle two outer rings contain 7 alpha subunits which form the PRTS gate.  The two inner rings contain 6 beta subunits with protease activity.  The 19S cap is composed of a base with 10 proteins six of which are ATPases and a lid which contains 9 proteins which binds polyubiquitin. The images at the left and at the right correspond to one representative Proteasome, ''i.e.'' the crystal structure of yeast Proteasome ([[3gpt]]).
+The core particle two outer rings contain 7 α subunits (Y7, Y13, PRE6, PRE5, PUP2, C1, C7-α) which form the PRTS gate.  The two inner rings contain 7 β subunits (PRE2, PRE4, PRE3, PUP1, PUP3, C5, C11) with protease activity<ref>PMID:8811196</ref>.
+*<scene name='41/417543/Cv/2'>α subunits and β subunits</scene>.
-{{TOC limit|limit=2}}
+*<scene name='41/417543/Cv/6'>Salinosporamide derivative binding site</scene> of chain H.
+*<scene name='41/417543/Cv/7'>Covalent bond between Salinosporamide derivative and Thr1</scene>.
 == 3D Structures of Proteasome ==
+[[Proteasome 3D structures]]
+== See Also ==
+[[PROTAC]] (Proteolysis-Targeting Chimera), an alternative to conventional enzyme inhibitors, by enabling proteasome induced degradation of the target protein.
+</StructureSection>
+== References ==
-=== 20S particle ===
+<references/>
-[[3mfe]], [[3mi0]], [[2fhg]] – Mt20S core – ''Mycobacterium tuberculosis''<br />
-[[3gpt]], [[3gpw]], [[1g0u]] – y20S core - yeast<br />
-[[1ryp]] - y20S core (mutant)<br />
-[[3mka]], [[3hfa]] – Mt20S core (mutant)<br />
-[[3c91]], [[3c92]], [[1pma]] – Ta20S core - ''Themoplasma acidophilum''<br />
-[[3h4p]] – 20S core – ''Methanocaldococcus janaschii''<br />
-[[2h6j]], [[1q5r]] – Re20S core (mutant) – ''Rhodococcus erythropolis''<br />
-[[1q5q]] - Re20S core<br />
-[[1j2q]] – Af20S core - ''Archaeoglobus fulgidus''<br />
-[[1iru]] – 20S core - bovine<br />
-=== 20S+activator protein ===
-[[1vsy]], [[1l5q]] – y20S core+ Blm10 <br />
-[[1z7q]], [[1fnt]] – y20S core+PA26<br />
-[[3ipm]], [[3jrm]], [[3jse]], [[3jtl]], [[1ya7]], [[1yar]], [[1yau]] - Taa-PRTS chains A-G+ Tab-PRTS chains H-N+PA26 chains O-U <br />
-=== 20S+inhibitor ===
-[[3hye]], [[3gpj]], [[3dy3]], [[3dy4]], [[3e47]], [[3d29]], [[3bdm]], [[2gpl]], [[2fak]], [[2f16]], [[1jd2]], [[1g65]] – y20S core+inhibitor<br />
-[[3h6f]], [[3h6i]], [[3hf9]], [[2fhh]] – Mt20S core+inhibitor<br />
-=== Partial 20S ===
-[[2ku1]], [[2ku2]] – Taa-PRTS chains A-G <br />
-[[3fp9]] – MtATPase chains A-L<br />
-[[1j2p]] – Af α subunit<br />
 [[Category:Topic Page]]