3px7: Difference between revisions
New page: '''Unreleased structure''' The entry 3px7 is ON HOLD Authors: Zhang, Zhongtao, Tse-dinh, YC, Cheng, B. Description: Crystal Structure of covalent complex of topoisomerase 1A with subst... |
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The | ==Crystal Structure of covalent complex of topoisomerase 1A with substrate== | ||
<StructureSection load='3px7' size='340' side='right'caption='[[3px7]], [[Resolution|resolution]] 2.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3px7]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_DH1 Escherichia coli DH1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PX7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PX7 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=PTR:O-PHOSPHOTYROSINE'>PTR</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3px7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3px7 OCA], [https://pdbe.org/3px7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3px7 RCSB], [https://www.ebi.ac.uk/pdbsum/3px7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3px7 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/TOP1_ECOLI TOP1_ECOLI] Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.<ref>PMID:9497321</ref> <ref>PMID:10681504</ref> <ref>PMID:21482796</ref> | |||
==See Also== | |||
*[[Topoisomerase 3D structures|Topoisomerase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Escherichia coli DH1]] | |||
[[Category: Large Structures]] | |||
[[Category: Cheng B]] | |||
[[Category: Tse-dinh YC]] | |||
[[Category: Zhang Z]] | |||