Human Cardiac Troponin C: Difference between revisions
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{{STRUCTURE_2kdh|PDB=2kdh | | {{STRUCTURE_2kdh| PDB=2kdh | SIZE=300| SCENE=Human_Cardiac_Troponin_C/2kdh_noir/2|right| CAPTION=NMR structure of human troponin C showing Ca, complex with green tea polyphenol, [[2kdh]] }} | ||
[[Image:2kdh. | |||
[[Image:2kdh structure presentation.jpg|left|200px]] | |||
2KDH is the Solution Structure of Human Cardiac Troponin C in complex with the Green Tea Polyphenol : (-)-epigallocatechin-3-gallate. It is a 1 chain structure of sequence from [http://fr.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. That is the PDB name for the solution structure of Human Cardiac Troponin C in complex with the green tea Polyphenol (-)-epigallocatechin-3-gallate. | |||
Troponin C is a protein which can bind with calcium. That’s a subunit from a 3 subunited protein which is called Troponin. This protein is found in muscles. | Troponin C is a protein which can bind with calcium. That’s a subunit from a 3 subunited protein which is called Troponin. This protein is found in muscles. | ||
For example, heart muscle contraction is regulated by Ca2+ binding of troponin C to the thin filament.<ref>PMID:19542563</ref> In cardiovascular diseases, the myofilament response to Ca2+ is often altered. Compounds that rectify this perturbation are of considerable interest as therapeutics. Plant [http://en.wikipedia.org/wiki/Flavonoid flavonoids] have been found to provide protection against a variety of human illnesses such as cancers, infections, and heart diseases. (-)-Epigallocatechin gallate (EGCg), the prevalent flavonoid in green tea is one of those plant flavonoids. | For example, heart muscle contraction is regulated by Ca2+ binding of troponin C to the thin filament.<ref>PMID:19542563</ref> In cardiovascular diseases, the myofilament response to Ca2+ is often altered. Compounds that rectify this perturbation are of considerable interest as therapeutics. Plant [http://en.wikipedia.org/wiki/Flavonoid flavonoids] have been found to provide protection against a variety of human illnesses such as cancers, infections, and heart diseases. (-)-Epigallocatechin gallate (EGCg), the prevalent flavonoid in green tea is one of those plant flavonoids. | ||
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There are <scene name='Human_Cardiac_Troponin_C/Hydrophobic/2'>hydrophobic residues</scene> which form an hydrophobic pocket.EGCg interacts with AC3 hydrophobic domain of the molecule and binds with AC2 domain. But there are <scene name='Human_Cardiac_Troponin_C/Residues_of_beta-sheet/1'>some residues of beta-sheet</scene> wich have no interactions with that molecule and this suggets that the binding of EGCg is near of the hydrophobic pocket rather than deep within the pocket and it induces a small structural "opening". The opening degree of TnC is described by the inter-helical angles between helices E and F and between helices G and H. | There are <scene name='Human_Cardiac_Troponin_C/Hydrophobic/2'>hydrophobic residues</scene> which form an hydrophobic pocket.EGCg interacts with AC3 hydrophobic domain of the molecule and binds with AC2 domain. But there are <scene name='Human_Cardiac_Troponin_C/Residues_of_beta-sheet/1'>some residues of beta-sheet</scene> wich have no interactions with that molecule and this suggets that the binding of EGCg is near of the hydrophobic pocket rather than deep within the pocket and it induces a small structural "opening". The opening degree of TnC is described by the inter-helical angles between helices E and F and between helices G and H. | ||
[[Image:EGC- | [[Image:EGC-TnC2.jpg|300px|left|thumb|Figure 1: EGCg-TnC complex]] | ||
[[Image:Pocket surface.jpg| | [[Image:Pocket surface.jpg|300px|left|thumb|Figure 2: Pocket with a surface representation]] | ||
As said just before, EGCg makes contacts exclusively to hydrophobic residues that line the surface of TnC. Actually it binds near the surface of helix E, so near the N-terminus of TnC, with tetrahydropyran and benzenediol. The pyrogallol ring stays near the C-terminus of TnC, which explains the large chemical shift perturbations of some residues of the <scene name='Human_Cardiac_Troponin_C/Helix_h/1'>Helix H</scene>. | As said just before, EGCg makes contacts exclusively to hydrophobic residues that line the surface of TnC. Actually it binds near the surface of helix E, so near the N-terminus of TnC, with tetrahydropyran and benzenediol. The pyrogallol ring stays near the C-terminus of TnC, which explains the large chemical shift perturbations of some residues of the <scene name='Human_Cardiac_Troponin_C/Helix_h/1'>Helix H</scene>. | ||
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Green tea contains that EGCg polyphenol and it can then act as a modulator of heart contraction through its interaction with TnC. So the use of green tea can help in some heart diseases, and can be an alternative for a start for a treatment at home . | Green tea contains that EGCg polyphenol and it can then act as a modulator of heart contraction through its interaction with TnC. So the use of green tea can help in some heart diseases, and can be an alternative for a start for a treatment at home . | ||
==3D structures of troponin C== | |||
[[Troponin]] | |||
== References == | == References == | ||
<references/> | |||
[http://www.jbc.org/content/early/2009/06/20/jbc.M109.021352.full.pdf The journal of biological chemistry] | [http://www.jbc.org/content/early/2009/06/20/jbc.M109.021352.full.pdf The journal of biological chemistry] | ||