3npe: Difference between revisions

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-{{Seed}}
-[[Image:3npe.jpg|left|200px]]
-<!--
+==Structure of VP14 in complex with oxygen==
-The line below this paragraph, containing "STRUCTURE_3npe", creates the "Structure Box" on the page.
+<StructureSection load='3npe' size='340' side='right'caption='[[3npe]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3npe]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NPE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NPE FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=OH:HYDROXIDE+ION'>OH</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>
-{{STRUCTURE_3npe|  PDB=3npe  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3npe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3npe OCA], [https://pdbe.org/3npe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3npe RCSB], [https://www.ebi.ac.uk/pdbsum/3npe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3npe ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/NCED1_MAIZE NCED1_MAIZE] Has a 11,12(11',12') 9-cis epoxycarotenoid cleavage activity. Catalyzes the first step of abscisic-acid biosynthesis from carotenoids. Not active on the all-trans isomers of violaxanthin and neoxanthin. Contributes probably to abscisic acid synthesis for the induction of seed dormancy.<ref>PMID:9342392</ref> <ref>PMID:9188535</ref> <ref>PMID:20884803</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The key regulatory step in the biosynthesis of abscisic acid (ABA), a hormone central to the regulation of several important processes in plants, is the oxidative cleavage of the 11,12 double bond of a 9-cis-epoxycarotenoid. The enzyme viviparous14 (VP14) performs this cleavage in maize (Zea mays), making it a target for the rational design of novel chemical agents and genetic modifications that improve plant behavior through the modulation of ABA levels. The structure of VP14, determined to 3.2-A resolution, provides both insight into the determinants of regio- and stereospecificity of this enzyme and suggests a possible mechanism for oxidative cleavage. Furthermore, mutagenesis of the distantly related CCD1 of maize shows how the VP14 structure represents a template for all plant carotenoid cleavage dioxygenases (CCDs). In addition, the structure suggests how VP14 associates with the membrane as a way of gaining access to its membrane soluble substrate.
-===Structure of VP14 in complex with oxygen===
+Structural insights into maize viviparous14, a key enzyme in the biosynthesis of the phytohormone abscisic acid.,Messing SA, Gabelli SB, Echeverria I, Vogel JT, Guan JC, Tan BC, Klee HJ, McCarty DR, Amzel LM Plant Cell. 2010 Sep;22(9):2970-80. Epub 2010 Sep 30. PMID:20884803<ref>PMID:20884803</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_20884803}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 3npe" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 20884803 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_20884803}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-NPE is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NPE OCA].
-==Reference==
-<ref group="xtra">PMID:20884803</ref><references group="xtra"/>
-[[Category: 9-cis-epoxycarotenoid dioxygenase]]
 [[Category: Zea mays]]
-[[Category: Amzel, L M.]]
+[[Category: Amzel LM]]
-[[Category: Gabelli, S B.]]
+[[Category: Gabelli SB]]
-[[Category: Messing, S A.]]
+[[Category: Messing SA]]
-[[Category: Abscisic acid]]
-[[Category: Dioxygenase]]
-[[Category: Non heme iron]]
-[[Category: Oxidoreductase]]
-[[Category: Seven blade beta propeller]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 10 06:50:32 2010''