3k6y: Difference between revisions

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-{{Seed}}
-[[Image:3k6y.jpg|left|200px]]
-<!--
+==Crystal structure of Rv3671c protease from M. tuberculosis, active form==
-The line below this paragraph, containing "STRUCTURE_3k6y", creates the "Structure Box" on the page.
+<StructureSection load='3k6y' size='340' side='right'caption='[[3k6y]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3k6y]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K6Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3K6Y FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3k6y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k6y OCA], [https://pdbe.org/3k6y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3k6y RCSB], [https://www.ebi.ac.uk/pdbsum/3k6y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3k6y ProSAT]</span></td></tr>
-{{STRUCTURE_3k6y|  PDB=3k6y  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Y3671_MYCTU Y3671_MYCTU] Required for M.tuberculosis resistance to oxidative stress in addition to its role in resistance to acid, which is essential for virulence. It protects M.tuberculosis against phagolysosomal concentrations of acid and maintains its intrabacterial pH when phagocytosed by IFN-gamma-activated macrophages.<ref>PMID:18641659</ref> <ref>PMID:20947023</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Rv3671c, a putative serine protease, is crucial for persistence of Mycobacterium tuberculosis in the hostile environment of the phagosome. We show that Rv3671c is required for M. tuberculosis resistance to oxidative stress in addition to its role in protection from acidification. Structural and biochemical analyses demonstrate that the periplasmic domain of Rv3671c is a functional serine protease of the chymotrypsin family and, remarkably, that its activity increases on oxidation. High-resolution crystal structures of this protease in an active strained state and in an inactive relaxed state reveal that a solvent-exposed disulfide bond controls the protease activity by constraining two distant regions of Rv3671c and stabilizing it in the catalytically active conformation. In vitro biochemical studies confirm that activation of the protease in an oxidative environment is dependent on this reversible disulfide bond. These results suggest that the disulfide bond modulates activity of Rv3671c depending on the oxidative environment in vivo.
-===Crystal structure of Rv3671c protease from M. tuberculosis, active form===
+Structural insight into serine protease Rv3671c that Protects M. tuberculosis from oxidative and acidic stress.,Biswas T, Small J, Vandal O, Odaira T, Deng H, Ehrt S, Tsodikov OV Structure. 2010 Oct 13;18(10):1353-63. PMID:20947023<ref>PMID:20947023</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-==About this Structure==
+</div>
-K6Y is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K6Y OCA].
+<div class="pdbe-citations 3k6y" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Mycobacterium tuberculosis]]
-[[Category: Biswas, T.]]
+[[Category: Biswas T]]
-[[Category: Ehrt, S.]]
+[[Category: Ehrt S]]
-[[Category: Small, J.]]
+[[Category: Small J]]
-[[Category: Tsodikov, O V.]]
+[[Category: Tsodikov OV]]
-[[Category: Vandal, O.]]
+[[Category: Vandal O]]
-[[Category: Bent helix]]
-[[Category: Disulfide]]
-[[Category: Hydrolase]]
-[[Category: Oxidative stress]]
-[[Category: Protease]]
-[[Category: Serine protease]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 13 10:47:07 2010''