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==Structure of StnII-Y111N, a mutant of the sea anemone actinoporin Sticholysin II== | |||
<StructureSection load='2l2b' size='340' side='right'caption='[[2l2b]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2l2b]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Stichodactyla_helianthus Stichodactyla helianthus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L2B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L2B FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l2b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l2b OCA], [https://pdbe.org/2l2b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l2b RCSB], [https://www.ebi.ac.uk/pdbsum/2l2b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l2b ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/ACTP2_STIHL ACTP2_STIHL] Pore-forming protein that forms cations-selective hydrophilic pores of around 1 nm and causes cardiac stimulation and hemolysis. Pore formation is a multi-step process that involves specific recognition of membrane sphingomyelin (but neither cholesterol nor phosphatidylcholine) using aromatic rich region and adjacent phosphocholine (POC) binding site, firm binding to the membrane (mainly driven by hydrophobic interactions) accompanied by the transfer of the N-terminal region to the lipid-water interface and finally pore formation after oligomerization of several monomers. Cytolytic effects include red blood cells hemolysis, platelet aggregation and lysis, cytotoxic and cytostatic effects on fibroblasts. Lethality in mammals has been ascribed to severe vasospasm of coronary vessels, cardiac arrhythmia, and inotropic effects.<ref>PMID:10978735</ref> <ref>PMID:11478962</ref> | |||
==See Also== | |||
*[[Cytolysin 3D structures|Cytolysin 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Stichodactyla helianthus]] | |||
[[Category: Bruix M]] | |||
[[Category: Pardo-Cea MA]] | |||
[[Category: Santoro J]] | |||
Latest revision as of 09:51, 1 May 2024
Structure of StnII-Y111N, a mutant of the sea anemone actinoporin Sticholysin IIStructure of StnII-Y111N, a mutant of the sea anemone actinoporin Sticholysin II
Structural highlights
FunctionACTP2_STIHL Pore-forming protein that forms cations-selective hydrophilic pores of around 1 nm and causes cardiac stimulation and hemolysis. Pore formation is a multi-step process that involves specific recognition of membrane sphingomyelin (but neither cholesterol nor phosphatidylcholine) using aromatic rich region and adjacent phosphocholine (POC) binding site, firm binding to the membrane (mainly driven by hydrophobic interactions) accompanied by the transfer of the N-terminal region to the lipid-water interface and finally pore formation after oligomerization of several monomers. Cytolytic effects include red blood cells hemolysis, platelet aggregation and lysis, cytotoxic and cytostatic effects on fibroblasts. Lethality in mammals has been ascribed to severe vasospasm of coronary vessels, cardiac arrhythmia, and inotropic effects.[1] [2] See AlsoReferences
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