Proteasome: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Alexander Berchansky, Michal Harel, Joel L. Sussman, Jaime Prilusky

@@ Line 1: / Line 1: @@
-[[Image:3gpt.png|left|200px|thumb|Crystal Structure of Proteasome, [[3gpt]]]]
+<StructureSection load='' size='350' side='right' scene='41/417543/Cv/1' caption='Yeast proteasome 20S core complex with salinosporamide derivative, [[3gpt]]'>
-{{STRUCTURE_3gpt|  PDB=3gpt  | SIZE=300| SCENE= |right|CAPTION=Proteasome, [[3gpt]] }}
+== Function ==
+[[Proteasome]] (PRTS) are large protein complexes which degrade unneeded proteins into small polypeptides<ref>PMID:22350895</ref>.  The '''26S PRTS''' is composed of a central '''20S core particle''' which contains 4 stacked rings each with several members and two 19S caps.  The 19S cap is composed of a base with 10 proteins six of which are ATPases and a lid which contains 9 proteins which bind polyubiquitin. <br />
+*'''13S PRTS''' is a PRTS core intermediate containing α ring, partial β ring and 3 chaperones<ref>PMID:33846632</ref>.
+*'''15S PRTS''' is half of an 20S PRTS and upon dimerisation forms the mature 20S PRTS<ref>PMID:32442437</ref>.
+For more details see [[3unb]].
+== Structural highlights ==
-Proteasome (PRTS) are large protein complexes which degrade unneeded proteins into small polypeptides.  The 26S PRTS is composed of a central 20S core particle which contains 4 stacked rings each with several members and two 19S caps.  The core particle two outer rings contain 7 alpha subunits which form the PRTS gate.  The two inner rings contain 6 beta subunits with protease activity.  The 19S cap is composed of a base with 10 proteins six of which are ATPases and a lid which contains 9 proteins which binds polyubiquitin. The images at the left and at the right correspond to one representative Proteasome, ''i.e.'' the crystal structure of yeast Proteasome ([[3gpt]]).
+The core particle two outer rings contain 7 α subunits (Y7, Y13, PRE6, PRE5, PUP2, C1, C7-α) which form the PRTS gate.  The two inner rings contain 7 β subunits (PRE2, PRE4, PRE3, PUP1, PUP3, C5, C11) with protease activity<ref>PMID:8811196</ref>.
+*<scene name='41/417543/Cv/2'>α subunits and β subunits</scene>.
-{{TOC limit|limit=2}}
+*<scene name='41/417543/Cv/6'>Salinosporamide derivative binding site</scene> of chain H.
+*<scene name='41/417543/Cv/7'>Covalent bond between Salinosporamide derivative and Thr1</scene>.
 == 3D Structures of Proteasome ==
+[[Proteasome 3D structures]]
+== See Also ==
+[[PROTAC]] (Proteolysis-Targeting Chimera), an alternative to conventional enzyme inhibitors, by enabling proteasome induced degradation of the target protein.
+</StructureSection>
-S particle
+== References ==
+<references/>
-mfe, 3mi0, 2fhg – Mt20S core – Mycobacterium tuberculosis
+[[Category:Topic Page]]
-gpt, 3gpw, 1g0u – y20S core - yeast
-ryp - y20S core (mutant)
-mka, 3hfa – Mt20S core (mutant)
-c91, 3c92, 1pma – Ta20S core - Themoplasma acidophilum
-h4p – 20S core – Methanocaldococcus janaschii
-h6j, 1q5r – Re20S core (mutant) – Rhodococcus erythropolis
-q5q - Re20S core
-j2q – Af20S core - Archaeoglobus fulgidus
-iru – 20S core - bovine
-S+activator protein
-vsy, 1l5q – y20S core+ Blm10
-z7q, 1fnt – y20S core+PA26
-ipm, 3jrm, 3jse, 3jtl, 1ya7, 1yar, 1yau - Taa-PRTS chains A-G+ Tab-PRTS chains H-N+PA26 chains O-U
-S+inhibitor
-hye, 3gpj, 3dy3, 3dy4, 3e47, 3d29, 3zcy, 3bdm, 2gpl, 2fak, 2f16, 1jd2, 1g65 – y20S core+inhibitor
-h6f, 3h6i, 3hf9, 2fhh – Mt20S core+inhibitor
-Partial 20S
-ku1, 2ku2 – Taa-PRTS chains A-G
-fp9 – MtATPase chains A-L
-j2p – Af  subunit