3nrx: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-{{Seed}}
-[[Image:3nrx.png|left|200px]]
-<!--
+==Insights into anti-parallel microtubule crosslinking by PRC1, a conserved non-motor microtubule binding protein==
-The line below this paragraph, containing "STRUCTURE_3nrx", creates the "Structure Box" on the page.
+<StructureSection load='3nrx' size='340' side='right'caption='[[3nrx]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3nrx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NRX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NRX FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nrx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nrx OCA], [https://pdbe.org/3nrx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nrx RCSB], [https://www.ebi.ac.uk/pdbsum/3nrx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nrx ProSAT]</span></td></tr>
-{{STRUCTURE_3nrx|  PDB=3nrx  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/PRC1_HUMAN PRC1_HUMAN] Key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Required to recruit PLK1 to the spindle. Stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle.<ref>PMID:9885575</ref> <ref>PMID:12082078</ref> <ref>PMID:15297875</ref> <ref>PMID:15625105</ref> <ref>PMID:16431929</ref> <ref>PMID:19468300</ref> <ref>PMID:20691902</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nr/3nrx_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3nrx ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Formation of microtubule architectures, required for cell shape maintenance in yeast, directional cell expansion in plants and cytokinesis in eukaryotes, depends on antiparallel microtubule crosslinking by the conserved MAP65 protein family. Here, we combine structural and single molecule fluorescence methods to examine how PRC1, the human MAP65, crosslinks antiparallel microtubules. We find that PRC1's microtubule binding is mediated by a structured domain with a spectrin-fold and an unstructured Lys/Arg-rich domain. These two domains, at each end of a homodimer, are connected by a linkage that is flexible on single microtubules, but forms well-defined crossbridges between antiparallel filaments. Further, we show that PRC1 crosslinks are compliant and do not substantially resist filament sliding by motor proteins in vitro. Together, our data show how MAP65s, by combining structural flexibility and rigidity, tune microtubule associations to establish crosslinks that selectively "mark" antiparallel overlap in dynamic cytoskeletal networks.
-===Insights into anti-parallel microtubule crosslinking by PRC1, a conserved non-motor microtubule binding protein===
+Insights into antiparallel microtubule crosslinking by PRC1, a conserved nonmotor microtubule binding protein.,Subramanian R, Wilson-Kubalek EM, Arthur CP, Bick MJ, Campbell EA, Darst SA, Milligan RA, Kapoor TM Cell. 2010 Aug 6;142(3):433-43. PMID:20691902<ref>PMID:20691902</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_20691902}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 3nrx" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 20691902 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_20691902}}
+__TOC__
+</StructureSection>
-==About this Structure==
-NRX is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NRX OCA].
-==Reference==
-<ref group="xtra">PMID:20691902</ref><references group="xtra"/>
 [[Category: Homo sapiens]]
-[[Category: Arthur, C P.]]
+[[Category: Large Structures]]
-[[Category: Bick, M J.]]
+[[Category: Arthur CP]]
-[[Category: Campbell, E A.]]
+[[Category: Bick MJ]]
-[[Category: Darst, S A.]]
+[[Category: Campbell EA]]
-[[Category: Kapoor, T M.]]
+[[Category: Darst SA]]
-[[Category: Milligan, R A.]]
+[[Category: Kapoor TM]]
-[[Category: Subramanian, R.]]
+[[Category: Milligan RA]]
-[[Category: Wilson-Kubalek, E M.]]
+[[Category: Subramanian R]]
-[[Category: Microtubule binding domain]]
+[[Category: Wilson-Kubalek EM]]
-[[Category: Protein binding]]
-[[Category: Spectrin fold]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 25 08:18:18 2010''