2kfy: Difference between revisions

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-{{Seed}}
-[[Image:2kfy.jpg|left|200px]]
-<!--
+==NMR structure of the first qRRM of hnRNP F in complex with AGGGAU G-tract RNA==
-The line below this paragraph, containing "STRUCTURE_2kfy", creates the "Structure Box" on the page.
+<StructureSection load='2kfy' size='340' side='right'caption='[[2kfy]]' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2kfy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KFY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KFY FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kfy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kfy OCA], [https://pdbe.org/2kfy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kfy RCSB], [https://www.ebi.ac.uk/pdbsum/2kfy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kfy ProSAT]</span></td></tr>
-{{STRUCTURE_2kfy|  PDB=2kfy  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/HNRPF_HUMAN HNRPF_HUMAN] Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state.<ref>PMID:20526337</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kf/2kfy_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kfy ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The heterogeneous nuclear ribonucleoprotein (hnRNP) F is involved in the regulation of mRNA metabolism by specifically recognizing G-tract RNA sequences. We have determined the solution structures of the three quasi-RNA-recognition motifs (qRRMs) of hnRNP F in complex with G-tract RNA. These structures show that qRRMs bind RNA in a very unusual manner, with the G-tract 'encaged', making the qRRM a novel RNA binding domain. We defined a consensus signature sequence for qRRMs and identified other human qRRM-containing proteins that also specifically recognize G-tract RNAs. Our structures explain how qRRMs can sequester G-tracts, maintaining them in a single-stranded conformation. We also show that isolated qRRMs of hnRNP F are sufficient to regulate the alternative splicing of the Bcl-x pre-mRNA, suggesting that hnRNP F would act by remodeling RNA secondary and tertiary structures.
-===NMR structure of the first qRRM of hnRNP F in complex with AGGGAU G-tract RNA===
+Structural basis of G-tract recognition and encaging by hnRNP F quasi-RRMs.,Dominguez C, Fisette JF, Chabot B, Allain FH Nat Struct Mol Biol. 2010 Jul;17(7):853-61. Epub 2010 Jun 6. PMID:20526337<ref>PMID:20526337</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2kfy" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-KFY is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KFY OCA].
+*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Allain, F H.T.]]
+[[Category: Large Structures]]
-[[Category: Dominguez, C.]]
+[[Category: Allain FHT]]
-[[Category: Acetylation]]
+[[Category: Dominguez C]]
-[[Category: G tract]]
-[[Category: Mrna processing]]
-[[Category: Mrna splicing]]
-[[Category: Nucleus]]
-[[Category: Phosphoprotein]]
-[[Category: Polyadenylation regulation]]
-[[Category: Polymorphism]]
-[[Category: Protein-rna complex]]
-[[Category: Ribonucleoprotein]]
-[[Category: Rna binding protein-rna complex]]
-[[Category: Rna-binding]]
-[[Category: Spliceosome]]
-[[Category: Splicing regulation]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 10 11:10:32 2010''