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< | ==Solution NMR structure of an O6-methylguanine DNA methyltransferase family protein from Vibrio parahaemolyticus. Northeast Structural Genomics Consortium target VpR247.== | ||
<StructureSection load='2kif' size='340' side='right'caption='[[2kif]]' scene=''> | |||
You may | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2kif]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_parahaemolyticus_AQ3810 Vibrio parahaemolyticus AQ3810]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KIF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KIF FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kif FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kif OCA], [https://pdbe.org/2kif PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kif RCSB], [https://www.ebi.ac.uk/pdbsum/2kif PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kif ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/ATL_VIBPQ ATL_VIBPQ] Involved in DNA damage recognition. Binds DNA containing O(6)-methylguanine (PubMed:20212037). Binds to the damaged base and flips the base out of the DNA duplex into an extrahelical conformation, which allows processing by repair proteins (By similarity).[UniProtKB:P0AFP2]<ref>PMID:20212037</ref> | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ki/2kif_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kif ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Alkyltransferase-like proteins (ATLs) are a novel class of DNA repair proteins related to O(6)-alkylguanine-DNA alkyltransferases (AGTs) that tightly bind alkylated DNA and shunt the damaged DNA into the nucleotide excision repair pathway. Here, we present the first structure of a bacterial ATL, from Vibrio parahaemolyticus (vpAtl). We demonstrate that vpAtl adopts an AGT-like fold and that the protein is capable of tightly binding to O(6)-methylguanine-containing DNA and disrupting its repair by human AGT, a hallmark of ATLs. Mutation of highly conserved residues Tyr(23) and Arg(37) demonstrate their critical roles in a conserved mechanism of ATL binding to alkylated DNA. NMR relaxation data reveal a role for conformational plasticity in the guanine-lesion recognition cavity. Our results provide further evidence for the conserved role of ATLs in this primordial mechanism of DNA repair. | |||
Structural basis of O6-alkylguanine recognition by a bacterial alkyltransferase-like DNA repair protein.,Aramini JM, Tubbs JL, Kanugula S, Rossi P, Ertekin A, Maglaqui M, Hamilton K, Ciccosanti CT, Jiang M, Xiao R, Soong TT, Rost B, Acton TB, Everett JK, Pegg AE, Tainer JA, Montelione GT J Biol Chem. 2010 Apr 30;285(18):13736-41. Epub 2010 Mar 8. PMID:20212037<ref>PMID:20212037</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2kif" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[DNA methyltransferase 3D structures|DNA methyltransferase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
== | [[Category: Large Structures]] | ||
[[Category: Vibrio parahaemolyticus AQ3810]] | |||
[[Category: Acton TB]] | |||
== | [[Category: Aramini JM]] | ||
< | [[Category: Belote RL]] | ||
[[Category: Vibrio parahaemolyticus | [[Category: Ciccosanti CT]] | ||
[[Category: Acton | [[Category: Everett JK]] | ||
[[Category: Aramini | [[Category: Jiang M]] | ||
[[Category: Belote | [[Category: Montelione GT]] | ||
[[Category: Ciccosanti | [[Category: Nair R]] | ||
[[Category: Everett | [[Category: Rost B]] | ||
[[Category: Jiang | [[Category: Swapna GVT]] | ||
[[Category: Montelione | [[Category: Xiao R]] | ||
[[Category: Nair | |||
[[Category: Rost | |||
[[Category: Swapna | |||
[[Category: Xiao | |||
Latest revision as of 12:39, 22 May 2024
Solution NMR structure of an O6-methylguanine DNA methyltransferase family protein from Vibrio parahaemolyticus. Northeast Structural Genomics Consortium target VpR247.Solution NMR structure of an O6-methylguanine DNA methyltransferase family protein from Vibrio parahaemolyticus. Northeast Structural Genomics Consortium target VpR247.
Structural highlights
FunctionATL_VIBPQ Involved in DNA damage recognition. Binds DNA containing O(6)-methylguanine (PubMed:20212037). Binds to the damaged base and flips the base out of the DNA duplex into an extrahelical conformation, which allows processing by repair proteins (By similarity).[UniProtKB:P0AFP2][1] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedAlkyltransferase-like proteins (ATLs) are a novel class of DNA repair proteins related to O(6)-alkylguanine-DNA alkyltransferases (AGTs) that tightly bind alkylated DNA and shunt the damaged DNA into the nucleotide excision repair pathway. Here, we present the first structure of a bacterial ATL, from Vibrio parahaemolyticus (vpAtl). We demonstrate that vpAtl adopts an AGT-like fold and that the protein is capable of tightly binding to O(6)-methylguanine-containing DNA and disrupting its repair by human AGT, a hallmark of ATLs. Mutation of highly conserved residues Tyr(23) and Arg(37) demonstrate their critical roles in a conserved mechanism of ATL binding to alkylated DNA. NMR relaxation data reveal a role for conformational plasticity in the guanine-lesion recognition cavity. Our results provide further evidence for the conserved role of ATLs in this primordial mechanism of DNA repair. Structural basis of O6-alkylguanine recognition by a bacterial alkyltransferase-like DNA repair protein.,Aramini JM, Tubbs JL, Kanugula S, Rossi P, Ertekin A, Maglaqui M, Hamilton K, Ciccosanti CT, Jiang M, Xiao R, Soong TT, Rost B, Acton TB, Everett JK, Pegg AE, Tainer JA, Montelione GT J Biol Chem. 2010 Apr 30;285(18):13736-41. Epub 2010 Mar 8. PMID:20212037[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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