2cfi: Difference between revisions
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== | ==The hydrolase domain of human 10-FTHFD in complex with 6- formyltetrahydropterin== | ||
<StructureSection load='2cfi' size='340' side='right'caption='[[2cfi]], [[Resolution|resolution]] 1.85Å' scene=''> | |||
[[ | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2cfi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CFI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CFI FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZZZ:6-FORMYLTETRAHYDROPTERIN'>ZZZ</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cfi OCA], [https://pdbe.org/2cfi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cfi RCSB], [https://www.ebi.ac.uk/pdbsum/2cfi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cfi ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/AL1L1_HUMAN AL1L1_HUMAN] | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cf/2cfi_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cfi ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
10-Formyltetrahydrofolate dehydrogenase is a ubiquitously expressed enzyme in the human body. It catalyses the formation of tetrahydrofolate and carbon dioxide from 10-formyltetrahydrofolate, thereby playing an important role in the human metabolism of one-carbon units. It is a two-domain protein in which the N-terminal domain hydrolyses 10-formyltetrahydrofolate into formate and tetrahydrofolate. The high-resolution crystal structure of the hydrolase domain from human 10-formyltetrahydrofolate dehydrogenase has been determined in the presence and absence of a substrate analogue. The structures reveal conformational changes of two loops upon ligand binding, while key active-site residues appear to be pre-organized for catalysis prior to substrate binding. Two water molecules in the structures mark the positions of key oxygen moieties in the catalytic reaction and reaction geometries are proposed based on the structural data. | |||
Structures of the hydrolase domain of human 10-formyltetrahydrofolate dehydrogenase and its complex with a substrate analogue.,Kursula P, Schuler H, Flodin S, Nilsson-Ehle P, Ogg DJ, Savitsky P, Nordlund P, Stenmark P Acta Crystallogr D Biol Crystallogr. 2006 Nov;62(Pt 11):1294-9. Epub 2006, Oct 18. PMID:17057331<ref>PMID:17057331</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2cfi" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Aldehyde dehydrogenase 3D structures|Aldehyde dehydrogenase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Arrowsmith | [[Category: Arrowsmith C]] | ||
[[Category: Edwards | [[Category: Edwards A]] | ||
[[Category: Ehn | [[Category: Ehn M]] | ||
[[Category: Graslund | [[Category: Graslund S]] | ||
[[Category: Hallberg | [[Category: Hallberg M]] | ||
[[Category: Hammarstrom | [[Category: Hammarstrom M]] | ||
[[Category: Kotenyova | [[Category: Kotenyova T]] | ||
[[Category: Kursula | [[Category: Kursula P]] | ||
[[Category: Nilsson-Ehle | [[Category: Nilsson-Ehle P]] | ||
[[Category: Nordlund | [[Category: Nordlund P]] | ||
[[Category: Ogg | [[Category: Ogg DJ]] | ||
[[Category: Persson | [[Category: Persson C]] | ||
[[Category: Sagemark | [[Category: Sagemark J]] | ||
[[Category: Schuler | [[Category: Schuler H]] | ||
[[Category: Stenmark | [[Category: Stenmark P]] | ||
[[Category: Sundstrom | [[Category: Sundstrom M]] | ||
[[Category: Thorsell | [[Category: Thorsell A]] | ||
[[Category: Weigelt | [[Category: Weigelt J]] | ||