2byu: Difference between revisions

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[[Image:2byu.png|left|200px]]


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==Negative stain EM reconstruction of M.tuberculosis Acr1(Hsp 16.3) fitted with wheat sHSP dimer==
The line below this paragraph, containing "STRUCTURE_2byu", creates the "Structure Box" on the page.
<SX load='2byu' size='340' side='right' viewer='molstar' caption='[[2byu]], [[Resolution|resolution]] 16.50&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2byu]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BYU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BYU FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 16.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2byu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2byu OCA], [https://pdbe.org/2byu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2byu RCSB], [https://www.ebi.ac.uk/pdbsum/2byu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2byu ProSAT]</span></td></tr>
{{STRUCTURE_2byu|  PDB=2byu  |  SCENE=  }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/HS16B_WHEAT HS16B_WHEAT]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/by/2byu_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2byu ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Small heat shock proteins are a ubiquitous and diverse family of stress proteins that have in common an alpha-crystallin domain. Mycobacterium tuberculosis has two small heat shock proteins, Acr1 (alpha-crystallin-related protein 1, or Hsp16.3/16-kDa antigen) and Acr2 (HrpA), both of which are highly expressed under different stress conditions. Small heat shock proteins form large oligomeric assemblies and are commonly polydisperse. Nanoelectrospray mass spectrometry showed that Acr2 formed a range of oligomers composed of dimers and tetramers, whereas Acr1 was a dodecamer. Electron microscopy of Acr2 showed a variety of particle sizes. Using three-dimensional analysis of negative stain electron microscope images, we have shown that Acr1 forms a tetrahedral assembly with 12 polypeptide chains. The atomic structure of a related alpha-crystallin domain dimer was docked into the density to build a molecular structure of the dodecameric Acr1 complex. Along with the differential regulation of these two proteins, the differences in their quaternary structures demonstrated here supports their distinct functional roles.


===NEGATIVE STAIN EM RECONSTRUCTION OF M.TUBERCULOSIS ACR1(HSP 16.3) FITTED WITH WHEAT SHSP DIMER===
Dodecameric structure of the small heat shock protein Acr1 from Mycobacterium tuberculosis.,Kennaway CK, Benesch JL, Gohlke U, Wang L, Robinson CV, Orlova EV, Saibil HR, Keep NH J Biol Chem. 2005 Sep 30;280(39):33419-25. Epub 2005 Jul 26. PMID:16046399<ref>PMID:16046399</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2byu" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_16046399}}, adds the Publication Abstract to the page
*[[Heat Shock Protein structures|Heat Shock Protein structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 16046399 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_16046399}}
__TOC__
 
</SX>
==About this Structure==
[[Category: Large Structures]]
2BYU is a 12 chains structure with sequences from [http://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BYU OCA].
 
==Reference==
<ref group="xtra">PMID:16046399</ref><references group="xtra"/>
[[Category: Triticum aestivum]]
[[Category: Triticum aestivum]]
[[Category: Benesch, J L.P.]]
[[Category: Benesch JLP]]
[[Category: Gohlke, U.]]
[[Category: Gohlke U]]
[[Category: Keep, N H.]]
[[Category: Keep NH]]
[[Category: Kennaway, C K.]]
[[Category: Kennaway CK]]
[[Category: Orlova, E V.]]
[[Category: Orlova EV]]
[[Category: Robinson, C V.]]
[[Category: Robinson CV]]
[[Category: Saibil, H R.]]
[[Category: Saibil HR]]
[[Category: Wang, L.]]
[[Category: Wang L]]
[[Category: Alpha-crystallin]]
[[Category: Chaperone]]
[[Category: Small heat shock protein]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 21 08:52:26 2010''

Latest revision as of 12:20, 9 May 2024

Negative stain EM reconstruction of M.tuberculosis Acr1(Hsp 16.3) fitted with wheat sHSP dimerNegative stain EM reconstruction of M.tuberculosis Acr1(Hsp 16.3) fitted with wheat sHSP dimer

2byu, resolution 16.50Å

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OCA
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