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< | ==MODEL OF A FATTY ACID BINDING PROTEIN FROM ASCARIS SUUM, THEORETICAL MODEL== | ||
<StructureSection load='1as1' size='340' side='right'caption='[[1as1]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AS1 FirstGlance]. <br> | |||
or | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1as1 FirstGlance], [https://www.ebi.ac.uk/pdbsum/1as1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1as1 ProSAT]</span></td></tr> | ||
- | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Early development of the parasitic nematode, Ascaris suum, occurs inside a highly resistant eggshell, and the developing larva is bathed in perivitelline fluid. Two-dimensional gel analysis of perivitelline fluid from infective larvae reveals seven major proteins; a cDNA encoding one of these, As-p18, has been cloned, sequenced, and protein expressed in Escherichia coli. The predicted amino acid sequence of As-p18 exhibits similarities to the intracellular lipid-binding protein (iLBP) family including retinoid- and fatty acid-binding proteins (FABP). As-p18 is unusual in that it possesses a hydrophobic leader that is not present in the mature protein, the developmental regulation of its expression, and in terms of its predicted structure. Recombinant As-p18 is a functional FABP with a high affinity for both a fluorescent fatty acid analog (11(((5-(dimethylamino)-1-naphthalenyl)sulfonyl)amino) undecanoic acid) and oleic acid, but not retinol. Circular dichroism of rAs-p18 reveals a high beta-sheet content (62%), which is consistent with secondary structure for the protein predicted from sequence algorithms, and the structure of iLBPs. Unusual features are apparent in a structural model of As-p18 generated from existing crystal structures of iLBPs. As-p18 is not found in unembryonated eggs, begins to be synthesized at about day 3 of development, reaches a maximal concentration with the formation of the first-stage larva and remains abundant in the perivitelline fluid of the second-stage larva. Since As-p18 is not present in the post-infective third-stage larva or adult worm tissues, it appears to be exclusive to the egg. Surprisingly, however, Northern blot analysis yields mRNA for As-p18 not only in the early larval stages, but also the unembryonated egg, third-stage larvae, and ovaries of adult worms, even though the protein is not detectable from any of those sources. As-p18 may play a role in sequestering potentially toxic fatty acids and their peroxidation products, or it may be involved in the maintenance of the impermeable lipid layer of the eggshell. | |||
Secretion of a novel, developmentally regulated fatty acid-binding protein into the perivitelline fluid of the parasitic nematode, Ascaris suum.,Mei B, Kennedy MW, Beauchamp J, Komuniecki PR, Komuniecki R J Biol Chem. 1997 Apr 11;272(15):9933-41. PMID:9092532<ref>PMID:9092532</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 1as1" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
== | [[Category: Theoretical Model]] | ||
[[Category: Large Structures]] | |||
== | |||
< | |||
[[Category: Beauchamp, J]] | [[Category: Beauchamp, J]] | ||
[[Category: Kennedy, M W]] | [[Category: Kennedy, M W]] | ||
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[[Category: Komuniecki, R]] | [[Category: Komuniecki, R]] | ||
[[Category: Mei, B]] | [[Category: Mei, B]] | ||