1z3x: Difference between revisions

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-[[Image:1z3x.gif|left|200px]]<br /><applet load="1z3x" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1z3x, resolution 1.50&Aring;" />
-'''Structure of Gun4 from Thermosynechococcus elongatus'''<br />
-==Overview==
+==Structure of Gun4 from Thermosynechococcus elongatus==
-Gun4 has been implicated in a developmental signaling pathway between the, chloroplast and the nucleus involving magnesium protoporphyrin IX, (MgP(IX)), the first dedicated intermediate in the chlorophyll, biosynthetic pathway. Here we present the crystal structure of, Thermosynechococcus elongatus Gun4 at 1.5 A, describe the binding, affinities of Gun4 for substrate and product porphyrin molecules, and, identify a likely (Mg)P(IX) binding site on the protein. Kinetic analyses, show that Gun4 dramatically increases the efficiency of transformation of, porphyrin substrate to metalloporphyrin product and that it also reduces, the threshold Mg2+ concentration required for activity at low porphyrin, concentration. Gun4 therefore controls magnesium chelatase at, physiologically significant Mg2+ concentrations and likely acts as a, molecular switch in vivo so that in its absence magnesium chelatase is, inactive. This mechanism could allow Gun4 to mediate magnesium, protoporphyrin levels both for chlorophyll biosynthesis and for signaling, to the nucleus.
+<StructureSection load='1z3x' size='340' side='right'caption='[[1z3x]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1z3x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermosynechococcus_vestitus Thermosynechococcus vestitus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z3X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z3X FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z3x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z3x OCA], [https://pdbe.org/1z3x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z3x RCSB], [https://www.ebi.ac.uk/pdbsum/1z3x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z3x ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q8DIJ1_THEVB Q8DIJ1_THEVB]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z3/1z3x_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1z3x ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Gun4 has been implicated in a developmental signaling pathway between the chloroplast and the nucleus involving magnesium protoporphyrin IX (MgP(IX)), the first dedicated intermediate in the chlorophyll biosynthetic pathway. Here we present the crystal structure of Thermosynechococcus elongatus Gun4 at 1.5 A, describe the binding affinities of Gun4 for substrate and product porphyrin molecules, and identify a likely (Mg)P(IX) binding site on the protein. Kinetic analyses show that Gun4 dramatically increases the efficiency of transformation of porphyrin substrate to metalloporphyrin product and that it also reduces the threshold Mg2+ concentration required for activity at low porphyrin concentration. Gun4 therefore controls magnesium chelatase at physiologically significant Mg2+ concentrations and likely acts as a molecular switch in vivo so that in its absence magnesium chelatase is inactive. This mechanism could allow Gun4 to mediate magnesium protoporphyrin levels both for chlorophyll biosynthesis and for signaling to the nucleus.
-==About this Structure==
+Structural and biochemical characterization of Gun4 suggests a mechanism for its role in chlorophyll biosynthesis.,Davison PA, Schubert HL, Reid JD, Iorg CD, Heroux A, Hill CP, Hunter CN Biochemistry. 2005 May 31;44(21):7603-12. PMID:15909975<ref>PMID:15909975</ref>
-Z3X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermosynechococcus_elongatus Thermosynechococcus elongatus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Z3X OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-Structural and biochemical characterization of Gun4 suggests a mechanism for its role in chlorophyll biosynthesis., Davison PA, Schubert HL, Reid JD, Iorg CD, Heroux A, Hill CP, Hunter CN, Biochemistry. 2005 May 31;44(21):7603-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15909975 15909975]
+</div>
-[[Category: Single protein]]
+<div class="pdbe-citations 1z3x" style="background-color:#fffaf0;"></div>
-[[Category: Thermosynechococcus elongatus]]
+== References ==
-[[Category: Davison, P.A.]]
+<references/>
-[[Category: Hill, C.P.]]
+__TOC__
-[[Category: Hunter, C.N.]]
+</StructureSection>
-[[Category: Iorg, C.D.]]
+[[Category: Large Structures]]
-[[Category: Reid, J.D.]]
+[[Category: Thermosynechococcus vestitus]]
-[[Category: Robinson, H.]]
+[[Category: Davison PA]]
-[[Category: Schubert, H.L.]]
+[[Category: Hill CP]]
-[[Category: transferase activator]]
+[[Category: Hunter CN]]
+[[Category: Iorg CD]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 04:43:51 2007''
+[[Category: Reid JD]]
+[[Category: Robinson H]]
+[[Category: Schubert HL]]