1i9b: Difference between revisions

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New page: left|200px<br /><applet load="1i9b" size="450" color="white" frame="true" align="right" spinBox="true" caption="1i9b, resolution 2.7Å" /> '''X-RAY STRUCTURE OF AC...
 
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[[Image:1i9b.gif|left|200px]]<br /><applet load="1i9b" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1i9b, resolution 2.7&Aring;" />
'''X-RAY STRUCTURE OF ACETYLCHOLINE BINDING PROTEIN (ACHBP)'''<br />


==Overview==
==X-RAY STRUCTURE OF ACETYLCHOLINE BINDING PROTEIN (ACHBP)==
Pentameric ligand gated ion-channels, or Cys-loop receptors, mediate rapid, chemical transmission of signals. This superfamily of allosteric, transmembrane proteins includes the nicotinic acetylcholine (nAChR), serotonin 5-HT3, gamma-aminobutyric-acid (GABAA and GABAC) and glycine, receptors. Biochemical and electrophysiological information on the, prototypic nAChRs is abundant but structural data at atomic resolution, have been missing. Here we present the crystal structure of molluscan, acetylcholine-binding protein (AChBP), a structural and functional, homologue of the amino-terminal ligand-binding domain of an nAChR, alpha-subunit. In the AChBP homopentamer, the protomers have an, immunoglobulin-like topology. Ligand-binding sites are located at each of, five subunit interfaces and contain residues contributed by biochemically, determined 'loops' A to F. The subunit interfaces are highly variable, within the ion-channel family, whereas the conserved residues stabilize, the protomer fold. This AChBP structure is relevant for the development of, drugs against, for example, Alzheimer's disease and nicotine addiction.
<StructureSection load='1i9b' size='340' side='right'caption='[[1i9b]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1i9b]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Lymnaea_stagnalis Lymnaea stagnalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I9B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I9B FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i9b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i9b OCA], [https://pdbe.org/1i9b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i9b RCSB], [https://www.ebi.ac.uk/pdbsum/1i9b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i9b ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ACHP_LYMST ACHP_LYMST] Binds to acetylcholine. Modulates neuronal synaptic transmission.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i9/1i9b_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i9b ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1I9B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lymnaea_stagnalis Lymnaea stagnalis] with CA and EPE as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1I9B OCA].
*[[Acetylcholine binding protein 3D structures|Acetylcholine binding protein 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Crystal structure of an ACh-binding protein reveals the ligand-binding domain of nicotinic receptors., Brejc K, van Dijk WJ, Klaassen RV, Schuurmans M, van Der Oost J, Smit AB, Sixma TK, Nature. 2001 May 17;411(6835):269-76. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11357122 11357122]
[[Category: Large Structures]]
[[Category: Lymnaea stagnalis]]
[[Category: Lymnaea stagnalis]]
[[Category: Single protein]]
[[Category: Brejc K]]
[[Category: Brejc, K.]]
[[Category: Klaassen R]]
[[Category: Dijk, W.J.van.]]
[[Category: Schuurmans M]]
[[Category: Klaassen, R.]]
[[Category: Sixma TK]]
[[Category: Oost, J.van.der.]]
[[Category: Smit AB]]
[[Category: Schuurmans, M.]]
[[Category: Van Dijk WJ]]
[[Category: Sixma, T.K.]]
[[Category: Van der Oost J]]
[[Category: Smit, A.B.]]
[[Category: CA]]
[[Category: EPE]]
[[Category: igg fold]]
[[Category: pentamer]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 03:48:03 2007''

Latest revision as of 10:45, 3 April 2024

X-RAY STRUCTURE OF ACETYLCHOLINE BINDING PROTEIN (ACHBP)X-RAY STRUCTURE OF ACETYLCHOLINE BINDING PROTEIN (ACHBP)

Structural highlights

1i9b is a 5 chain structure with sequence from Lymnaea stagnalis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.7Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ACHP_LYMST Binds to acetylcholine. Modulates neuronal synaptic transmission.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1i9b, resolution 2.70Å

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