3f7m: Difference between revisions
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< | ==Crystal structure of apo Cuticle-Degrading Protease (ver112) from Verticillium psalliotae== | ||
<StructureSection load='3f7m' size='340' side='right'caption='[[3f7m]], [[Resolution|resolution]] 1.60Å' scene=''> | |||
You may | == Structural highlights == | ||
or the | <table><tr><td colspan='2'>[[3f7m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lecanicillium_psalliotae Lecanicillium psalliotae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F7M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3F7M FirstGlance]. <br> | ||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3f7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3f7m OCA], [https://pdbe.org/3f7m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3f7m RCSB], [https://www.ebi.ac.uk/pdbsum/3f7m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3f7m ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/ALP_LECPS ALP_LECPS] Serine protease which can degrade the nematode cuticle.<ref>PMID:16132863</ref> | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f7/3f7m_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3f7m ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Cuticle-degrading proteases are involved in the breakdown of cuticle/eggshells of nematodes or insects, a hard physical barrier against fungal infections. Understanding the 3-dimensional structures of these proteins can provide crucial information for improving the effectiveness of these fungi in biocontrol applications, e.g., by targeted protein engineering. However, the structures of these proteases remain unknown. Here, we report the structures of two cuticle-degrading proteases from two species of nematophagous fungi. The two structures were solved with X-ray crystallography to resolutions of 1.65 A (Ver112) and 2.1 A (PL646), respectively. Crystal structures of PL646 and Ver112 were found to be very similar to each other, and similar to that of proteinase K from another fungus Tritirachium album. Differences between the structures were found among residues of the substrate binding sites (S1 and S4). Experimental studies showed that the enzymes differed in hydrolytic activity to synthetic peptide substrates. Our analyses of the hydrophobic/hydrophilic and electrostatic features of these two proteins suggest that their surfaces likely play important roles during fungal infection against nematodes. The two crystal structures provide a solid basis for investigating the relationship between structure and function of cuticle-degrading proteases. | |||
The crystal structures of two cuticle-degrading proteases from nematophagous fungi and their contribution to infection against nematodes.,Liang L, Meng Z, Ye F, Yang J, Liu S, Sun Y, Guo Y, Mi Q, Huang X, Zou C, Rao Z, Lou Z, Zhang KQ FASEB J. 2010 May;24(5):1391-400. doi: 10.1096/fj.09-136408. Epub 2009 Dec 9. PMID:20007510<ref>PMID:20007510</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
== | </div> | ||
<div class="pdbe-citations 3f7m" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Lecanicillium psalliotae]] | [[Category: Lecanicillium psalliotae]] | ||
[[Category: Liang | [[Category: Liang L]] | ||
[[Category: Lou | [[Category: Lou Z]] | ||
[[Category: Meng | [[Category: Meng Z]] | ||
[[Category: Rao | [[Category: Rao Z]] | ||
[[Category: Ye | [[Category: Ye F]] | ||
[[Category: Zhang | [[Category: Zhang K]] | ||
Latest revision as of 12:07, 30 October 2024
Crystal structure of apo Cuticle-Degrading Protease (ver112) from Verticillium psalliotaeCrystal structure of apo Cuticle-Degrading Protease (ver112) from Verticillium psalliotae
Structural highlights
FunctionALP_LECPS Serine protease which can degrade the nematode cuticle.[1] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedCuticle-degrading proteases are involved in the breakdown of cuticle/eggshells of nematodes or insects, a hard physical barrier against fungal infections. Understanding the 3-dimensional structures of these proteins can provide crucial information for improving the effectiveness of these fungi in biocontrol applications, e.g., by targeted protein engineering. However, the structures of these proteases remain unknown. Here, we report the structures of two cuticle-degrading proteases from two species of nematophagous fungi. The two structures were solved with X-ray crystallography to resolutions of 1.65 A (Ver112) and 2.1 A (PL646), respectively. Crystal structures of PL646 and Ver112 were found to be very similar to each other, and similar to that of proteinase K from another fungus Tritirachium album. Differences between the structures were found among residues of the substrate binding sites (S1 and S4). Experimental studies showed that the enzymes differed in hydrolytic activity to synthetic peptide substrates. Our analyses of the hydrophobic/hydrophilic and electrostatic features of these two proteins suggest that their surfaces likely play important roles during fungal infection against nematodes. The two crystal structures provide a solid basis for investigating the relationship between structure and function of cuticle-degrading proteases. The crystal structures of two cuticle-degrading proteases from nematophagous fungi and their contribution to infection against nematodes.,Liang L, Meng Z, Ye F, Yang J, Liu S, Sun Y, Guo Y, Mi Q, Huang X, Zou C, Rao Z, Lou Z, Zhang KQ FASEB J. 2010 May;24(5):1391-400. doi: 10.1096/fj.09-136408. Epub 2009 Dec 9. PMID:20007510[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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