3f6j: Difference between revisions

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-{{Seed}}
-[[Image:3f6j.jpg|left|200px]]
-<!--
+==F17a-G lectin domain with bound GlcNAc(beta1-3)Gal==
-The line below this paragraph, containing "STRUCTURE_3f6j", creates the "Structure Box" on the page.
+<StructureSection load='3f6j' size='340' side='right'caption='[[3f6j]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3f6j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F6J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3F6J FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MBG:METHYL-BETA-GALACTOSE'>MBG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-{{STRUCTURE_3f6j|  PDB=3f6j  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3f6j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3f6j OCA], [https://pdbe.org/3f6j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3f6j RCSB], [https://www.ebi.ac.uk/pdbsum/3f6j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3f6j ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/F17AG_ECOLX F17AG_ECOLX] Essential fimbrial adhesion factor that mediates binding to N-acetylglucosamine-containing receptors in the host intestinal microvilli, leading to colonization of the intestinal tissue, and diarrhea or septicemia. Also confers adhesiveness to laminin and basement membranes.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Fimbriae are long, proteinaceous adhesion organelles expressed on the bacterial envelope, evolutionarily adapted by Escherichia coli strains for the colonization of epithelial linings. Using glycan arrays of the Consortium for Functional Glycomics (CFG), the lectin domains were screened of the fimbrial adhesins F17G and FedF from enterotoxigenic E. coli (ETEC) and of the FimH adhesin from uropathogenic E. coli. This has led to the discovery of a more specific receptor for F17G, GlcNAcb1,3Gal. No significant differences emerged from the glycan binding profiles of the F17G lectin domains from five different E. coli strains. However, strain-dependent amino acid variations, predominantly towards the positively charged arginine, were indicated by sulfate binding in FedF and F17G crystal structures. For FedF, no significant binders could be observed on the CFG glycan array. Hence, a shotgun array was generated from microvilli scrapings of the distal jejunum of a 3-week old piglet about to be weaned. On this array, the blood group A type 1 hexasaccharide emerged as a receptor for the FedF lectin domain and remarkably also for F18-fimbriated E. coli. F17G was found to selectively recognize glycan species with a terminal GlcNAc, typifying intestinal mucins. In conclusion, F17G and FedF recognize long glycan sequences that could only be identified using the shotgun approach. Interestingly, ETEC strains display a large capacity to adapt their fimbrial adhesins to ecological niches via charge-driven interactions, congruent with binding to thick mucosal surfaces displaying an acidic gradient along the intestinal tract.
-===F17a-G lectin domain with bound GlcNAc(beta1-3)Gal===
+Structural Sampling of Glycan Interaction Profiles Reveals Mucosal Receptors for Fimbrial Adhesins of Enterotoxigenic Escherichia coli.,Lonardi E, Moonens K, Buts L, de Boer AR, Olsson JD, Weiss MS, Fabre E, Guerardel Y, Deelder AM, Oscarson S, Wuhrer M, Bouckaert J Biology (Basel). 2013 Jul 1;2(3):894-917. doi: 10.3390/biology2030894. PMID:24833052<ref>PMID:24833052</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3f6j" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-F6J is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F6J OCA].
+*[[Adhesin 3D structures|Adhesin 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Escherichia coli]]
-[[Category: Boer, A de.]]
+[[Category: Large Structures]]
-[[Category: Bouckaert, J.]]
+[[Category: Bouckaert J]]
-[[Category: Buts, L.]]
+[[Category: Buts L]]
-[[Category: Genst, E De.]]
+[[Category: De Genst E]]
-[[Category: Greve, H De.]]
+[[Category: De Greve H]]
-[[Category: Guerardel, Y.]]
+[[Category: De Kerpel M]]
-[[Category: Jonckheere, W.]]
+[[Category: Guerardel Y]]
-[[Category: Kerpel, M De.]]
+[[Category: Jonckheere W]]
-[[Category: Olsson, J D.M.]]
+[[Category: Olsson JDM]]
-[[Category: Oscarson, S.]]
+[[Category: Oscarson S]]
-[[Category: Willaert, R.]]
+[[Category: Willaert R]]
-[[Category: Wuhrer, M.]]
+[[Category: Wuhrer M]]
-[[Category: Wyns, L.]]
+[[Category: Wyns L]]
-[[Category: Bacterial adhesion]]
+[[Category: De Boer A]]
-[[Category: Bacterial attachment]]
-[[Category: Cell projection]]
-[[Category: Fimbrium]]
-[[Category: Immunoglobulin fold]]
-[[Category: Lectin]]
-[[Category: Pathogenesis]]
-[[Category: Sugar binding protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 18 20:01:09 2009''