1lpw: Difference between revisions

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New page: left|200px<br /><applet load="1lpw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lpw" /> '''Solution structure of the yeast spliceosomal...
 
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[[Image:1lpw.gif|left|200px]]<br /><applet load="1lpw" size="450" color="white" frame="true" align="right" spinBox="true"
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'''Solution structure of the yeast spliceosomal U2 snRNA-intron branch site helix featuring a conserved pseudouridine'''<br />


==Overview==
==Solution structure of the yeast spliceosomal U2 snRNA-intron branch site helix featuring a conserved pseudouridine==
Pairing of a consensus sequence of the precursor (pre)-mRNA intron with a, short region of the U2 small nuclear (sn)RNA during assembly of the, eukaryotic spliceosome results in formation of a complementary helix of, seven base pairs with a single unpaired adenosine residue. The 2' OH of, this adenosine, called the branch site, brings about nucleophilic attack, at the pre-mRNA 5' splice site in the first step of splicing. Another, feature of this pairing is the phylogenetic conservation of a, pseudouridine (psi) residue in U2 snRNA nearly opposite the branch site., We show that the presence of this psi in the pre-mRNA branch-site helix of, Saccharomyces cerevisiae induces a dramatically altered architectural, landscape compared with that of its unmodified counterpart. The, psi-induced structure places the nucleophile in an accessible position for, the first step of splicing.
<StructureSection load='1lpw' size='340' side='right'caption='[[1lpw]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1lpw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LPW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LPW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PSU:PSEUDOURIDINE-5-MONOPHOSPHATE'>PSU</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lpw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lpw OCA], [https://pdbe.org/1lpw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lpw RCSB], [https://www.ebi.ac.uk/pdbsum/1lpw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lpw ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Pairing of a consensus sequence of the precursor (pre)-mRNA intron with a short region of the U2 small nuclear (sn)RNA during assembly of the eukaryotic spliceosome results in formation of a complementary helix of seven base pairs with a single unpaired adenosine residue. The 2' OH of this adenosine, called the branch site, brings about nucleophilic attack at the pre-mRNA 5' splice site in the first step of splicing. Another feature of this pairing is the phylogenetic conservation of a pseudouridine (psi) residue in U2 snRNA nearly opposite the branch site. We show that the presence of this psi in the pre-mRNA branch-site helix of Saccharomyces cerevisiae induces a dramatically altered architectural landscape compared with that of its unmodified counterpart. The psi-induced structure places the nucleophile in an accessible position for the first step of splicing.


==About this Structure==
Sculpting of the spliceosomal branch site recognition motif by a conserved pseudouridine.,Newby MI, Greenbaum NL Nat Struct Biol. 2002 Dec;9(12):958-65. PMID:12426583<ref>PMID:12426583</ref>
1LPW is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LPW OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Sculpting of the spliceosomal branch site recognition motif by a conserved pseudouridine., Newby MI, Greenbaum NL, Nat Struct Biol. 2002 Dec;9(12):958-65. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12426583 12426583]
</div>
[[Category: Protein complex]]
<div class="pdbe-citations 1lpw" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Greenbaum, N.L.]]
[[Category: Greenbaum NL]]
[[Category: Newby, M.I.]]
[[Category: Newby MI]]
[[Category: branch site]]
[[Category: nmr]]
[[Category: pseudouridine]]
[[Category: u2 snrna]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 03:14:36 2007''

Latest revision as of 21:48, 29 November 2023

Solution structure of the yeast spliceosomal U2 snRNA-intron branch site helix featuring a conserved pseudouridineSolution structure of the yeast spliceosomal U2 snRNA-intron branch site helix featuring a conserved pseudouridine

Structural highlights

1lpw is a 2 chain structure with sequence from Saccharomyces cerevisiae. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Pairing of a consensus sequence of the precursor (pre)-mRNA intron with a short region of the U2 small nuclear (sn)RNA during assembly of the eukaryotic spliceosome results in formation of a complementary helix of seven base pairs with a single unpaired adenosine residue. The 2' OH of this adenosine, called the branch site, brings about nucleophilic attack at the pre-mRNA 5' splice site in the first step of splicing. Another feature of this pairing is the phylogenetic conservation of a pseudouridine (psi) residue in U2 snRNA nearly opposite the branch site. We show that the presence of this psi in the pre-mRNA branch-site helix of Saccharomyces cerevisiae induces a dramatically altered architectural landscape compared with that of its unmodified counterpart. The psi-induced structure places the nucleophile in an accessible position for the first step of splicing.

Sculpting of the spliceosomal branch site recognition motif by a conserved pseudouridine.,Newby MI, Greenbaum NL Nat Struct Biol. 2002 Dec;9(12):958-65. PMID:12426583[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. ↑ Newby MI, Greenbaum NL. Sculpting of the spliceosomal branch site recognition motif by a conserved pseudouridine. Nat Struct Biol. 2002 Dec;9(12):958-65. PMID:12426583 doi:10.1038/nsb873
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