User:Alicia Daeden/Sandbox 130: Difference between revisions

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== '''The Solution Structure of Human Cardiac Troponin C in complex with the Green Tea Polyphenol; (-)-epigallocatechin-3-gallate''' ==

== '''2KDH : The Solution Structure of Human Cardiac Troponin C in complex with the Green Tea Polyphenol; (-)-epigallocatechin-3-gallate''' ==

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or leave the SCENE parameter empty for the default display.

-->

2KDH is a 1 chain structure of sequence from [http://fr.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. That is the PDB name for the solution structure of Human Cardiac Troponin C in complex with the green tea Polyphenol (-)-epigallocatechin-3-gallate.

Troponin C is a protein which can bind with calcium. That’s a subunit from a 3 subunited protein which is called Troponin. This protein is found in muscles.

For example, heart muscle contraction is regulated by Ca2+ binding of troponin C to the thin filament. In cardiovascular disease, the myofilament response to Ca2+ is often altered. Compounds that rectify this perturbation are of considerable interest as therapeutics. Plant [http://en.wikipedia.org/wiki/Flavonoid flavonoids] have been found to provide protection against a variety of human illnesses such as cancer, infection, and heart disease. (-)-Epigallocatechin gallate (EGCg), the prevalent flavonoid in green tea is one of those plant flavonoids.

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[[Image:EGC-TnC.jpg|350px]] '''''EGCg-TnC complex'''''

As said just before, EGCg makes contacts exclusively to hydrophobic residues that line the surface of TnC. Actually it binds near the surface of helix E, so near the N-terminus of TnC, with tetrahydropyran and benzenediol. The pyrogallol ring stays near the C-terminus of TnC, which explains the large chemical shift perturbations of some residues of the <scene name='User:Alicia_Daeden/Sandbox_130/Helix_h/1'>helix H</scene>

As said just before, EGCg makes contacts exclusively to hydrophobic residues that line the surface of TnC. Actually it binds near the surface of helix E, so near the N-terminus of TnC, with tetrahydropyran and benzenediol. The pyrogallol ring stays near the C-terminus of TnC, which explains the large chemical shift perturbations of some residues of the <scene name='User:Alicia_Daeden/Sandbox_130/Helix_h/1'>helix H</scene>.

Moreover, EGCg can bind the TnC-2Ca2+ or TnC-2Ca2+-TnI and forms a ternary complex, which increases the binding potential.

Moreover, EGCg can bind with the TnC-2Ca2+ or TnC-2Ca2+-TnI and forms a ternary complex, which increases the binding potential.

== Therapy application ==

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Common treatment schemes of heart failure modify levels of cytosolic Ca2+. This provides immediate improvement in heart function, but it can lead to serious side effects if used for an extended period of time. Drugs that alter the Ca2+ sensitivity of the thin filament rather than the cytosolic Ca2+ concentration, provide a safer alternative.

An increase in Ca2+ sensitivity would be beneficial for the treatment of heart failure, whereas the use of Ca2+ desentizers may provide protection against the development of hypertrophic cardiomyopathy (HCM).

EGCg has a role as Ca2+ desensitizer and is particularly interesting in regards to treatment for HCM because that is a scavenger of radicals and this may help treat or prevent HCM by sequestering reactive oxygen species as well as by inhibiting ATPases activity (this prevents binding of Ca2+ so there is no ATPase activity).

Green tea contains that EGCg polyphenol and it can then act as a modulator of heart contraction through its interaction with TnC. So the use of green tea can help in some heart diseases, ~~(and could be involved in the development of new drugs, ça fait bizarre ici)~~ and can be an alternative for a start for a treatment at home .

Green tea contains that EGCg polyphenol and it can then act as a modulator of heart contraction through its interaction with TnC. So the use of green tea can help in some heart diseases, and can be an alternative for a start for a treatment at home .

== References ==

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[http://www.rcsb.org/pdb/explore/explore.do?structureId=2KDH PDB]

[http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KDH OCA]

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-== '''The Solution Structure of Human Cardiac Troponin C in complex with the Green Tea Polyphenol; (-)-epigallocatechin-3-gallate''' ==
+== '''2KDH : The Solution Structure of Human Cardiac Troponin C in complex with the Green Tea Polyphenol; (-)-epigallocatechin-3-gallate''' ==
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 or leave the SCENE parameter empty for the default display.
 -->
-{{STRUCTURE_2kdh|  PDB=2kdh  |  SCENE=  }}
+{{STRUCTURE_2kdh|  PDB=2kdh  |  SCENE | background-color:#D8D8D8 }}
-KDH is a 1 chain structure of sequence from [http://fr.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. That is the PDB name for the solution structure of Human Cardiac Troponin C in complex with the green tea Polyphenol (-)-epigallocatechin-3-gallate.
+KDH is a 1 chain structure of sequence from [http://fr.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. That is the PDB name for the solution  structure of Human Cardiac Troponin C in complex with the green tea Polyphenol (-)-epigallocatechin-3-gallate.
 Troponin C is a protein which can bind with calcium. That’s a subunit from a 3 subunited protein which is called Troponin. This protein is found in muscles.
 For example, heart muscle contraction is regulated by Ca2+ binding of troponin C to the thin filament. In cardiovascular disease, the myofilament response to Ca2+ is often altered. Compounds that rectify this perturbation are of considerable interest as therapeutics. Plant [http://en.wikipedia.org/wiki/Flavonoid flavonoids] have been found to provide protection against a variety of human illnesses such as cancer, infection, and heart disease. (-)-Epigallocatechin gallate (EGCg), the prevalent flavonoid in green tea is one of those plant flavonoids.
@@ Line 34: / Line 34: @@
 [[Image:EGC-TnC.jpg|350px]] '''''EGCg-TnC complex'''''
-As said just before, EGCg makes contacts exclusively to hydrophobic residues that line the surface of TnC. Actually it binds near the surface of helix E, so near the N-terminus of TnC, with tetrahydropyran and benzenediol. The pyrogallol ring stays near the C-terminus of TnC, which explains the large chemical shift perturbations of some residues of the <scene name='User:Alicia_Daeden/Sandbox_130/Helix_h/1'>helix H</scene>
+As said just before, EGCg makes contacts exclusively to hydrophobic residues that line the surface of TnC. Actually it binds near the surface of helix E, so near the N-terminus of TnC, with tetrahydropyran and benzenediol. The pyrogallol ring stays near the C-terminus of TnC, which explains the large chemical shift perturbations of some residues of the <scene name='User:Alicia_Daeden/Sandbox_130/Helix_h/1'>helix H</scene>.
-Moreover, EGCg can bind the TnC-2Ca2+ or TnC-2Ca2+-TnI and forms a ternary complex, which increases the binding potential.
+Moreover, EGCg can bind with the TnC-2Ca2+ or TnC-2Ca2+-TnI and forms a ternary complex, which increases the binding potential.
 == Therapy application ==
@@ Line 44: / Line 44: @@
 Common treatment schemes of heart failure modify levels of cytosolic Ca2+. This provides immediate improvement in heart function, but it can lead to serious side effects if used for an extended period of time. Drugs that alter the Ca2+ sensitivity of the thin filament rather than the cytosolic Ca2+ concentration, provide a safer alternative.
 An increase in Ca2+ sensitivity would be beneficial for the treatment of heart failure, whereas the use of Ca2+ desentizers may provide protection against the development of hypertrophic cardiomyopathy (HCM).
 EGCg has a role as Ca2+ desensitizer and is particularly interesting in regards to treatment for HCM because that is a scavenger of radicals and this may help treat or prevent HCM by sequestering reactive oxygen species as well as by inhibiting ATPases activity (this prevents binding of Ca2+ so there is no ATPase activity).
-Green tea contains that EGCg polyphenol and it can then act as a modulator of heart contraction through its interaction with TnC. So the use of green tea can help in some heart diseases, (and could be involved in the development of new drugs, ça fait bizarre ici) and can be an alternative for a start for a treatment at home .
+Green tea contains that EGCg polyphenol and it can then act as a modulator of heart contraction through its interaction with TnC. So the use of green tea can help in some heart diseases, and can be an alternative for a start for a treatment at home .
 == References ==
@@ Line 53: / Line 55: @@
 [http://www.rcsb.org/pdb/explore/explore.do?structureId=2KDH PDB]
+[http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KDH OCA]