1d58: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
New page: left|200px<br /><applet load="1d58" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d58, resolution 1.700Å" /> '''THE MOLECULAR STRUC...
 
No edit summary
 
(15 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1d58.gif|left|200px]]<br /><applet load="1d58" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1d58, resolution 1.700&Aring;" />
'''THE MOLECULAR STRUCTURE OF A 4'-EPIADRIAMYCIN COMPLEX WITH D(TGATCA) AT 1.7 ANGSTROM RESOLUTION-COMPARISON WITH THE STRUCTURE OF 4'-EPIADRIAMYCIN D(TGTACA) AND D(CGATCG) COMPLEXES'''<br />


==Overview==
==THE MOLECULAR STRUCTURE OF A 4'-EPIADRIAMYCIN COMPLEX WITH D(TGATCA) AT 1.7 ANGSTROM RESOLUTION-COMPARISON WITH THE STRUCTURE OF 4'-EPIADRIAMYCIN D(TGTACA) AND D(CGATCG) COMPLEXES==
The structure of the complex between d(TGATCA) and the anthracycline, 4'-epiadriamycin has been determined by crystallographic methods. The, crystals are tetragonal, space group P4(1)2(1)2 with unit cell dimensions, of a = 28.01, c = 52.95A. The asymmetric unit consists of one strand of, hexanucleotide, one molecule of 4'-epiadriamycin and 34 waters. The, R-factor is 20.2% for 1694 reflections with F greater than or equal to 2, sigma F to 1.7A. Two asymmetric units associate to generate a duplex, complexed with two drug molecules at the d(TpG) steps of the duplex. The, chromophore intercalates between these base pairs with the anthracycline, amino-sugar positioned in the minor groove. The double helix is a, distorted B-DNA type structure. Our structure determination of d(TGATCA), complexed to 4'-epiadriamycin allows for comparison with the previously, reported structures of 4'-epiadriamycin bound to d(TGTACA) and to, d(CGATCG). The three complexes are similar in gross features and the, intercalation geometry is the same irrespective of whether a d(CpG) or, d(TpG) sequence is involved. However, the orientation of the amino-sugar, displays a dependence on the sequence adjacent to the intercalation site., The flexibility of this amino-sugar may help explain why this class of, antibiotics displays a relative insensitivity to base sequence when they, bind to DNA.
<StructureSection load='1d58' size='340' side='right'caption='[[1d58]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1d58]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D58 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D58 FirstGlance]. <br>
1D58 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with DM6 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1D58 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DM6:4-EPIDOXORUBICIN'>DM6</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d58 OCA], [https://pdbe.org/1d58 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d58 RCSB], [https://www.ebi.ac.uk/pdbsum/1d58 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d58 ProSAT]</span></td></tr>
The molecular structure of a 4'-epiadriamycin complex with d(TGATCA) at 1.7A resolution: comparison with the structure of 4'-epiadriamycin d(TGTACA) and d(CGATCG) complexes., Langlois d'Estaintot B, Gallois B, Brown T, Hunter WN, Nucleic Acids Res. 1992 Jul 25;20(14):3561-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1641324 1641324]
</table>
[[Category: Protein complex]]
__TOC__
[[Category: Brown, T.]]
</StructureSection>
[[Category: Estaintot, B.Langlois.D.]]
[[Category: Large Structures]]
[[Category: Gallois, B.]]
[[Category: Brown T]]
[[Category: Hunter, W.N.]]
[[Category: Gallois B]]
[[Category: DM6]]
[[Category: Hunter WN]]
[[Category: complexed with drug]]
[[Category: Langlois D'Estaintot B]]
[[Category: double helix]]
[[Category: right handed dna]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:31:30 2007''

Latest revision as of 09:49, 7 February 2024

THE MOLECULAR STRUCTURE OF A 4'-EPIADRIAMYCIN COMPLEX WITH D(TGATCA) AT 1.7 ANGSTROM RESOLUTION-COMPARISON WITH THE STRUCTURE OF 4'-EPIADRIAMYCIN D(TGTACA) AND D(CGATCG) COMPLEXESTHE MOLECULAR STRUCTURE OF A 4'-EPIADRIAMYCIN COMPLEX WITH D(TGATCA) AT 1.7 ANGSTROM RESOLUTION-COMPARISON WITH THE STRUCTURE OF 4'-EPIADRIAMYCIN D(TGTACA) AND D(CGATCG) COMPLEXES

Structural highlights

1d58 is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.7Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

1d58, resolution 1.70Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=1d58&oldid=4041594"