2wnf: Difference between revisions

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-{{Seed}}
-[[Image:2wnf.jpg|left|200px]]
-<!--
+==Crystal Structure of a Mammalian Sialyltransferase in complex with Gal-beta-1-3GalNAc-ortho-nitrophenol==
-The line below this paragraph, containing "STRUCTURE_2wnf", creates the "Structure Box" on the page.
+<StructureSection load='2wnf' size='340' side='right'caption='[[2wnf]], [[Resolution|resolution]] 1.25&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2wnf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WNF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WNF FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.25&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=CG3:HYDROXY(2-HYDROXYPHENYL)OXOAMMONIUM'>CG3</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PRD_900084:Thomsen-Friedenreich+antigen'>PRD_900084</scene></td></tr>
-{{STRUCTURE_2wnf|  PDB=2wnf  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wnf OCA], [https://pdbe.org/2wnf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wnf RCSB], [https://www.ebi.ac.uk/pdbsum/2wnf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wnf ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/SIA4A_PIG SIA4A_PIG]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wn/2wnf_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wnf ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Mammalian cell surfaces are modified by complex arrays of glycoproteins, glycolipids and polysaccharides, many of which terminate in sialic acid and have central roles in essential processes including cell recognition, adhesion and immunogenicity. Sialylation of glycoconjugates is performed by a set of sequence-related enzymes known as sialyltransferases (STs). Here we present the crystal structure of a mammalian ST, porcine ST3Gal-I, providing a structural basis for understanding the mechanism and specificity of these enzymes and for the design of selective inhibitors.
-===CRYSTAL STRUCTURE OF A MAMMALIAN SIALYLTRANSFERASE IN COMPLEX WITH GAL-BETA-1-3GALNAC-ORTHO-NITROPHENOL===
+Structural insight into mammalian sialyltransferases.,Rao FV, Rich JR, Rakic B, Buddai S, Schwartz MF, Johnson K, Bowe C, Wakarchuk WW, Defrees S, Withers SG, Strynadka NC Nat Struct Mol Biol. 2009 Nov;16(11):1186-8. Epub 2009 Oct 11. PMID:19820709<ref>PMID:19820709</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2wnf" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-WNF is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WNF OCA].
+*[[Sialyltransferase 3D structures|Sialyltransferase 3D structures]]
-[[Category: Beta-galactoside alpha-2,3-sialyltransferase]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Sus scrofa]]
-[[Category: Raikic, B.]]
+[[Category: Raikic B]]
-[[Category: Rao, F V.]]
+[[Category: Rao FV]]
-[[Category: Rich, J R.]]
+[[Category: Rich JR]]
-[[Category: Strynadka, N C.J.]]
+[[Category: Strynadka NCJ]]
-[[Category: Wakarchuk, W W.]]
+[[Category: Wakarchuk WW]]
-[[Category: Withers, S G.]]
+[[Category: Withers SG]]
-[[Category: Alternative splicing]]
-[[Category: Disulfide bond]]
-[[Category: Glycoprotein]]
-[[Category: Glycosyltransferase]]
-[[Category: Golgi apparatus]]
-[[Category: Membrane]]
-[[Category: Secreted]]
-[[Category: Sialic acid]]
-[[Category: Sialyltransferase]]
-[[Category: Signal-anchor]]
-[[Category: Transferase]]
-[[Category: Transmembrane]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 14 10:29:16 2009''