1ecp: Difference between revisions

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-[[Image:1ecp.gif|left|200px]]<br />
-<applet load="1ecp" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1ecp, resolution 2.0&Aring;" />
-'''PURINE NUCLEOSIDE PHOSPHORYLASE'''<br />
-==Overview==
+==PURINE NUCLEOSIDE PHOSPHORYLASE==
-BACKGROUND: Purine nucleoside phosphorylase (PNP) from Escherichia coli is, a hexameric enzyme that catalyzes the reversible phosphorolysis of 6-amino, and 6-oxopurine (2'-deoxy)ribonucleosides to the free base and, (2'-deoxy)ribose-1-phosphate. In contrast, human and bovine PNPs are, trimeric and accept only 6-oxopurine nucleosides as substrates. The, difference in the specificities of these two enzymes has been utilized in, gene therapy treatments in which certain prodrugs are cleaved by E. coli, PNP but not the human enzyme. The trimeric and hexameric PNPs show no, similarity in amino acid sequence, even though they catalyze the same, basic chemical reaction. Structural comparison of the active sites of, mammalian and E. coli PNPs would provide an improved basis for the design, of ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9351810 (full description)]]
+<StructureSection load='1ecp' size='340' side='right'caption='[[1ecp]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1ecp]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ECP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ECP FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ecp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ecp OCA], [https://pdbe.org/1ecp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ecp RCSB], [https://www.ebi.ac.uk/pdbsum/1ecp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ecp ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/DEOD_ECOLI DEOD_ECOLI] Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules.[HAMAP-Rule:MF_01627]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ec/1ecp_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ecp ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-ECP is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]]. Active as [[http://en.wikipedia.org/wiki/Transferase Transferase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.1 2.4.2.1]]. Structure known Active Sites: S1 and S2. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ECP OCA]].
+*[[Purine nucleoside phosphorylase 3D structures|Purine nucleoside phosphorylase 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-The crystal structure of Escherichia coli purine nucleoside phosphorylase: a comparison with the human enzyme reveals a conserved topology., Mao C, Cook WJ, Zhou M, Koszalka GW, Krenitsky TA, Ealick SE, Structure. 1997 Oct 15;5(10):1373-83. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9351810 9351810]
+[[Category: Escherichia coli K-12]]
-[[Category: Escherichia coli]]
+[[Category: Large Structures]]
-[[Category: Single protein]]
+[[Category: Ealick SE]]
-[[Category: Ealick, S.E.]]
+[[Category: Mao C]]
-[[Category: Mao, C.]]
-[[Category: glycosyltransferase]]
-[[Category: pentosyltransferase]]
-[[Category: purine nucleoside phosphorylase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 08:38:22 2007''