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User:Eric Martz/Sandbox 6: Difference between revisions

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ConfirmAccount, Editor, Tester, Administrators
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Eric Martz (talk | contribs)
ConfirmAccount, Editor, Tester, Administrators
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<applet size='400' frame='true' align='right' caption='Insert caption here'
<applet size='450' frame='true' align='right' caption='Insert caption here'
scene='User:Eric_Martz/Sandbox_6/2hu4_3cl0_aligned/2' />
scene='User:Eric_Martz/Sandbox_6/2hu4_3cl0_aligned/4' />


Tamiflu is shown as thick sticks.
H274Y ([[Amino Acids#Histidine|Histidine]] to [[Amino Acids#Tyrosine|Tyrosine]]) is the most common mutation conferring resistance to the drug Tamiflu in the neuraminidase N1 of Influenza A (e.g. H1N1, H5N1). Although resistant, this mutant N1 still binds Tamiflu weakly, and a crystal structure was obtained, [[3ckz]]. Below, this is compared to the wild type, [[2hu4]]], using structural alignment of a single protein chain:Tamiflu complex from each.


N1:
'''Why is the affinity of Tamiflu reduced by the mutation H274Y?''' Use the scenes below to help answer this question.
<jmol>
 
<jmolButton>
Tamiflu is shown as thick sticks. Atoms contacting Tamiflu (4 &Aring;) are shown as balls. Amino acids that contain contacting atoms, plus amino acid 274, are shown as thin sticks. All other amino acids are hidden.
<script>frame 1.1</script>
 
<text>Wild Type</text>
Influenza neuraminidase N1:
</jmolButton>
</jmol>


<jmol>
<jmol>
<jmolButton>
<jmolButton>
<script>frame 1.2</script>
<script>anim off; frame 1.2</script>
<text>H274Y</text>
<text>H274Y</text>
</jmolButton>
</jmolButton>
</jmol>
</jmol>
<jmol>
<jmol>
<jmolButton>
<jmolButton>
<script>if (~animation); if (_animating); anim pause;set echo bottom left; color echo white; font echo 20 sansserif;echo Animation Paused; else; anim mode loop 0 0;anim fps 2; anim resume; set echo off;endif;endif;</script>
<script>anim off; frame 1.1</script>
<text>Toggle Animation</text>
<text>Wild Type</text>
</jmolButton>
</jmolButton>
</jmol>
</jmol>
<jmol>
<jmol>
<jmolButton>
<jmolButton>
<script>if (~animation); if (_animating); anim pause;set echo bottom left; color echo white; font echo 20 sansserif;echo Animation Paused; else; anim mode loop 0 0;anim fps 2; anim resume; set echo off;endif;endif;</script>
<script>anim off; frame 0</script>
<text>Toggle Animation</text>
<text>Both</text>
</jmolButton>
</jmolButton>
</jmol>
</jmol>
<jmol>
<jmol>
<jmolButton>
<jmolButton>
<script>if (~animation); if (_animating); anim pause;set echo bottom left; color echo white; font echo 20 sansserif;echo Animation Paused; else; anim mode loop 0 0;anim fps 2; anim resume; set echo off;endif;endif;</script>
<script>if (~animation); if (_animating); anim pause; else; anim mode loop 0 0;anim fps 2; anim resume; endif; endif;</script>
<text>Toggle Animation</text>
<text>Toggle Animation</text>
</jmolButton>
</jmolButton>
</jmol>
</jmol>
Atoms are colored by element:
{{Template:ColorKey_Element_C}},
{{Template:ColorKey_Element_N}},
{{Template:ColorKey_Element_O}} (in protein),
<font color="magenta"><b>O</b></font> (in water).
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