Boris Brumshtein
Joined 28 October 2007
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[[Image:BorisBrumshtein-face-picture.png|thumb|right|190px Year:2007]] | [[Image:BorisBrumshtein-face-picture.png|thumb|right|190px| Year:2007]] | ||
==CV== | ==CV== | ||
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====Molecular biology and biochemistry==== | ====Molecular biology and biochemistry==== | ||
*Protein cloning and expression in E.Coli. | *Protein cloning and expression in E.Coli. | ||
*Biochemical and protein purification techniques: HPLC, FPLC, Ni-columns, Ion | *Biochemical and protein purification techniques: HPLC, FPLC, Ni-columns, Ion exchange columns, acryl-amide gels, size exclusion, affinity chromatography, gradient and analytical ultracentrifugation, ultra-filtration, Mini Prep cell, etc. | ||
exchange columns, acryl-amide gels, size exclusion, affinity chromatography, | *Drug-Protein interaction analysis employing various structural, biochemical and biophysical assays such as: enzymatic assays, ELIZA, circular dichroism, dynamic light scattering, mass-spec, BiaCore, analytical ultracentrifugation, etc. | ||
gradient and analytical ultracentrifugation, ultra-filtration, Mini Prep cell, etc. | |||
*Drug-Protein interaction analysis employing various structural, biochemical and | |||
biophysical assays such as: enzymatic assays, ELIZA, circular dichroism, dynamic | |||
light scattering, mass-spec, BiaCore, analytical ultracentrifugation, etc. | |||
====Programming languages==== | ====Programming languages==== | ||
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==LIST OF PUBLICATIONS== | ==LIST OF PUBLICATIONS== | ||
#Kacher Y, Brumshtein B, Boldin-Adamsky S, Toker L, Shainskaya A, Silman I, Sussman JL, Futerman AH. Acid-beta-glucosidase: insights from structural analysis and relevance to Gaucher disease therapy. Biol Chem. 2008 Sep 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18783340 18783340] | |||
#Harel M, Brumshtein B, Meged R, Dvir H, Ravelli RB, McCarthy A, Toker L, Silman I, Sussman JL. 3-D structure of serum paraoxonase 1 sheds light on its activity, stability, solubility and crystallizability. Arh Hig Rada Toksikol. 2007 Sep;58(3):347-53. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17913690 17913690] | #Harel M, Brumshtein B, Meged R, Dvir H, Ravelli RB, McCarthy A, Toker L, Silman I, Sussman JL. 3-D structure of serum paraoxonase 1 sheds light on its activity, stability, solubility and crystallizability. Arh Hig Rada Toksikol. 2007 Sep;58(3):347-53. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17913690 17913690] | ||
# Brumshtein B, Greenblatt HM, Butters TD, Shaaltiel Y, Aviezer D, Silman I, Futerman AH, Sussman JL. Crystal structures of complexes of N-butyl- and N-nonyl-deoxynojirimycin bound to acid-beta -glucosidase: Insights into the mechanism of chemical chaperone action in Gaucher disease. J.Biol. Chem., Vol. 282, Issue 39, 29052-29058, (2007). PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17666401 17666401] | # Brumshtein B, Greenblatt HM, Butters TD, Shaaltiel Y, Aviezer D, Silman I, Futerman AH, Sussman JL. Crystal structures of complexes of N-butyl- and N-nonyl-deoxynojirimycin bound to acid-beta -glucosidase: Insights into the mechanism of chemical chaperone action in Gaucher disease. J.Biol. Chem., Vol. 282, Issue 39, 29052-29058, (2007). PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17666401 17666401] | ||
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Last update [[User:Boris Brumshtein|Boris Brumshtein]] | The research is been performed in laboratories of [http://www.weizmann.ac.il/sb/faculty_pages/Sussman/ Prof. Joel L. Sussman], [http://www.weizmann.ac.il/neurobiology/faculty.html Prof. Israel Silman] and [http://www.weizmann.ac.il/Biological_Chemistry/scientist/futerman/ Prof. Tony H. Futerman], | ||
[http://www.weizmann.ac.il/ Weizmann Institute of Science] | |||
Last update [[User:Boris Brumshtein|Boris Brumshtein]] 00:00, 29 September 2008 (IDT) | |||