Institute of Clinical Biochemistry: Difference between revisions
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=About IKB= | |||
Institute of Clinical Biochemistry (IKB) is situated at the Rikshospitalet University Hospital, and is part of the University of Oslo (Norway) | |||
=Research at IKB= | =Research at IKB= | ||
[[1web.jpg]] | [[Image:1web.jpg|400px|thumb|Photo: Killian Munch]] | ||
Research at IKB aims to unravel the dynamic interplay between cellular processes on a molecular level, that can be used to understand and treat disease. Of particular interest are processes related to DNA integrity and mitochondrial biology. | Research at IKB aims to unravel the dynamic interplay between cellular processes on a molecular level, that can be used to understand and treat disease. Of particular interest are processes related to DNA integrity and mitochondrial biology. | ||
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==Research areas== | ==Research areas== | ||
===Mitochondria: DNA and function=== | ===Mitochondria: DNA and function=== | ||
The mitogroup focuses on function of mitochondria in relation to oxidative stress and mtDNA damage. Oxidative stress damages mtDNA but also suppresses respiratory function of mitochondria. Particular interest is the interplay between respiration function and mtDNA damage accumulation/repair. Molecular methods are used to characterize damage and repair of mtDNA, assembly of mitochondrial complexes in the mitochondrial inner membrane in addition to functional assessment by high-resolution respirometry and in vivo confocal imaging. | The mitogroup focuses on function of mitochondria in relation to oxidative stress and mtDNA damage. Oxidative stress damages mtDNA but also suppresses respiratory function of mitochondria. Particular interest is the interplay between respiration function and mtDNA damage accumulation/repair. Molecular methods are used to characterize damage and repair of mtDNA, assembly of mitochondrial complexes in the mitochondrial inner membrane in addition to functional assessment by high-resolution respirometry and in vivo confocal imaging. | ||
===DNA-repair: molecular- and structural biology of DNA base lesions=== | ===DNA-repair: molecular- and structural biology of DNA base lesions=== | ||
The DNA repair group focuses on how DNA base lesions are detected and processed by the various DNA repair proteins within the cell. We aim at mapping the molecular principles of recognition of specific base lesions that occur in DNA as a consequence of different processes like oxidation of DNA, damages by electromagnetic radiation, contact with reactive chemicals or different carcinogenic compounds. We use both biological and biochemical methods to study the function of single DNA repair proteins as well as the interplay between different proteins. Further, we also use methods within molecular biology like site-spesific mutagenesis and cloning in order to map the function and lesion specificity of the DNA repair proteins. We also take the advantage of structural biology (protein structure determination) and the optical laser tweezers to study the molecular interactions and dynamics of the different repair proteins. | The DNA repair group focuses on how DNA base lesions are detected and processed by the various DNA repair proteins within the cell. We aim at mapping the molecular principles of recognition of specific base lesions that occur in DNA as a consequence of different processes like oxidation of DNA, damages by electromagnetic radiation, contact with reactive chemicals or different carcinogenic compounds. We use both biological and biochemical methods to study the function of single DNA repair proteins as well as the interplay between different proteins. Further, we also use methods within molecular biology like site-spesific mutagenesis and cloning in order to map the function and lesion specificity of the DNA repair proteins. We also take the advantage of structural biology (protein structure determination) and the optical laser tweezers to study the molecular interactions and dynamics of the different repair proteins. | ||
===Core facility for structural biology=== | ===Core facility for structural biology=== | ||
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=Studies= | =Studies= | ||
[[Image:Oxygraph_study.JPG|300px|thumb|Photo: Killian Munch]] | |||
Academic staff at the institute is involved in PBL-based teaching. | Academic staff at the institute is involved in PBL-based teaching. | ||
Lars Eide 23071062; (lars.eide@rr-research.no) | *Lars Eide 23071062; (<email>lars.eide@rr-research.no</email>) | ||
Lars Mørkrid 23071075; (lars.morkrid@medisin.uio.no) | *Lars Mørkrid 23071075; (<email>lars.morkrid@medisin.uio.no</email>) | ||
==External Master Degree== | ==External Master Degree== | ||
We have previously supervised students who want to arrange an External Master Degree. In addition, we can accommodate students from "forskerlinjen" in medicine. | We have previously supervised students who want to arrange an External Master Degree. In addition, we can accommodate students from "forskerlinjen" in medicine. | ||