7ff8: Difference between revisions
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==Pseudomonas aeruginosa Virulence Factor Regulator with cAMP ligand and Cl(triethylphosphine)gold(I)== | |||
<StructureSection load='7ff8' size='340' side='right'caption='[[7ff8]], [[Resolution|resolution]] 2.80Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7ff8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FF8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FF8 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AU:GOLD+ION'>AU</scene>, <scene name='pdbligand=AUF:TRIETHYLPHOSPHANUIDYLGOLD(1+)'>AUF</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CMP:ADENOSINE-3,5-CYCLIC-MONOPHOSPHATE'>CMP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ff8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ff8 OCA], [https://pdbe.org/7ff8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ff8 RCSB], [https://www.ebi.ac.uk/pdbsum/7ff8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ff8 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/VFR_PSEAE VFR_PSEAE] Can bind cyclic AMP. Is a global regulator of virulence factor expression and is required for exotoxin A and protease production. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Pseudomonas aeruginosa is a leading cause of hospital-acquired infections. Treatment of P. aeruginosa infections is difficult given its multiple virulence mechanisms, intrinsic antibiotic resistance mechanisms, and biofilm-forming ability. Auranofin, an approved oral gold compound for rheumatoid arthritis treatment, was recently reported to inhibit the growth of multiple bacterial species. Here, we identify P. aeruginosa's global virulence factor regulator Vfr as one target of auranofin. We report the mechanistic insights into the inhibitory mechanism of auranofin and gold(I) analogues to Vfr through structural, biophysical, and phenotypic inhibition studies. This work suggests that auranofin and gold(I) analogues have potential to be developed as anti-virulence drugs against P. aeruginosa. | |||
Structural basis for the inhibitory mechanism of auranofin and gold(I) analogues against Pseudomonas aeruginosa global virulence factor regulator Vfr.,Zhang Y, Chew BLA, Wang J, Yuan M, Yam JKH, Luo D, Yang L Comput Struct Biotechnol J. 2023 Mar 13;21:2137-2146. doi: , 10.1016/j.csbj.2023.03.013. eCollection 2023. PMID:37007650<ref>PMID:37007650</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Chew | <div class="pdbe-citations 7ff8" style="background-color:#fffaf0;"></div> | ||
[[Category: Luo | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Pseudomonas aeruginosa PAO1]] | |||
[[Category: Chew BLA]] | |||
[[Category: Luo D]] | |||
Latest revision as of 11:27, 5 March 2025
Pseudomonas aeruginosa Virulence Factor Regulator with cAMP ligand and Cl(triethylphosphine)gold(I)Pseudomonas aeruginosa Virulence Factor Regulator with cAMP ligand and Cl(triethylphosphine)gold(I)
Structural highlights
FunctionVFR_PSEAE Can bind cyclic AMP. Is a global regulator of virulence factor expression and is required for exotoxin A and protease production. Publication Abstract from PubMedPseudomonas aeruginosa is a leading cause of hospital-acquired infections. Treatment of P. aeruginosa infections is difficult given its multiple virulence mechanisms, intrinsic antibiotic resistance mechanisms, and biofilm-forming ability. Auranofin, an approved oral gold compound for rheumatoid arthritis treatment, was recently reported to inhibit the growth of multiple bacterial species. Here, we identify P. aeruginosa's global virulence factor regulator Vfr as one target of auranofin. We report the mechanistic insights into the inhibitory mechanism of auranofin and gold(I) analogues to Vfr through structural, biophysical, and phenotypic inhibition studies. This work suggests that auranofin and gold(I) analogues have potential to be developed as anti-virulence drugs against P. aeruginosa. Structural basis for the inhibitory mechanism of auranofin and gold(I) analogues against Pseudomonas aeruginosa global virulence factor regulator Vfr.,Zhang Y, Chew BLA, Wang J, Yuan M, Yam JKH, Luo D, Yang L Comput Struct Biotechnol J. 2023 Mar 13;21:2137-2146. doi: , 10.1016/j.csbj.2023.03.013. eCollection 2023. PMID:37007650[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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