8xiu: Difference between revisions

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-'''Unreleased structure'''
-The entry 8xiu is ON HOLD  until Paper Publication
+==Cryo-EM structure of a frog VMAT2 in an apo conformation==
+<StructureSection load='8xiu' size='340' side='right'caption='[[8xiu]], [[Resolution|resolution]] 4.00&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[8xiu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8XIU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8XIU FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8xiu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8xiu OCA], [https://pdbe.org/8xiu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8xiu RCSB], [https://www.ebi.ac.uk/pdbsum/8xiu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8xiu ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Vesicular monoamine transporter 2 (VMAT2) belongs to the major facilitator superfamily (MFS), and mediates cytoplasmic monoamine packaging into presynaptic vesicles. Here, we present two cryo-EM structures of VMAT2, with a frog VMAT2 adopting a canonical MFS fold and an engineered sheep VMAT2 adopting a non-canonical fold. Both VMAT2 proteins mediate uptake of a selective fluorescent VMAT2 substrate into cells. Molecular docking, substrate binding and transport analysis reveal potential substrate binding mechanism in VMAT2. Meanwhile, caution is advised when interpreting engineered membrane protein structures.
-Authors:
+Engineering of a mammalian VMAT2 for cryo-EM analysis results in non-canonical protein folding.,Lyu Y, Fu C, Ma H, Su Z, Sun Z, Zhou X Nat Commun. 2024 Aug 2;15(1):6511. doi: 10.1038/s41467-024-50934-5. PMID:39095428<ref>PMID:39095428</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 8xiu" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Xenopus laevis]]
+[[Category: Fu C]]
+[[Category: Lyu Y]]
+[[Category: Ma H]]
+[[Category: Su Z]]
+[[Category: Sun Z]]
+[[Category: Zhou X]]