8wwb: Difference between revisions
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The | ==The sigma-1 receptor from Xenopus laevis in complex with dehydroepiandrosterone sulfate by soaking (I432 form)== | ||
<StructureSection load='8wwb' size='340' side='right'caption='[[8wwb]], [[Resolution|resolution]] 2.50Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8wwb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8WWB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8WWB FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.503Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZWY:17-oxoandrost-5-en-3beta-yl+hydrogen+sulfate'>ZWY</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8wwb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8wwb OCA], [https://pdbe.org/8wwb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8wwb RCSB], [https://www.ebi.ac.uk/pdbsum/8wwb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8wwb ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/SGMR1_XENLA SGMR1_XENLA] May function in lipid transport from the endoplasmic reticulum and be involved in a wide array of cellular functions probably through regulation of the biogenesis of lipid microdomains at the plasma membrane. May regulate calcium efflux at the endoplasmic reticulum (By similarity). | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The sigma-1 receptor (sigma1R) is a non-opioid membrane receptor, which responds to a diverse array of synthetic ligands to exert various pharmacological effects. Meanwhile, candidates for endogenous ligands of sigma1R have also been identified. However, how endogenous ligands bind to sigma1R remains unknown. Here, we present crystal structures of sigma1R from Xenopus laevis (xlsigma1R) bound to two endogenous neurosteroid ligands, progesterone (a putative antagonist) and dehydroepiandrosterone sulfate (DHEAS) (a putative agonist), at 2.15-3.09 A resolutions. Both neurosteroids bind to a similar location in xlsigma1R mainly through hydrophobic interactions, but surprisingly, with opposite binding orientations. DHEAS also forms hydrogen bonds with xlsigma1R, whereas progesterone interacts indirectly with the receptor through water molecules near the binding site. Binding analyses are consistent with the xlsigma1R-neurosteroid complex structures. Furthermore, molecular dynamics simulations and structural data reveal a potential water entry pathway. Our results provide insight into binding of two endogenous neurosteroid ligands to sigma1R. | |||
Insight into binding of endogenous neurosteroid ligands to the sigma-1 receptor.,Fu C, Xiao Y, Zhou X, Sun Z Nat Commun. 2024 Jul 4;15(1):5619. doi: 10.1038/s41467-024-49894-7. PMID:38965213<ref>PMID:38965213</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 8wwb" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Xenopus laevis]] | |||
[[Category: Fu C]] | |||
[[Category: Sun Z]] | |||
[[Category: Zhou X]] | |||
Latest revision as of 15:24, 17 July 2024
The sigma-1 receptor from Xenopus laevis in complex with dehydroepiandrosterone sulfate by soaking (I432 form)The sigma-1 receptor from Xenopus laevis in complex with dehydroepiandrosterone sulfate by soaking (I432 form)
Structural highlights
FunctionSGMR1_XENLA May function in lipid transport from the endoplasmic reticulum and be involved in a wide array of cellular functions probably through regulation of the biogenesis of lipid microdomains at the plasma membrane. May regulate calcium efflux at the endoplasmic reticulum (By similarity). Publication Abstract from PubMedThe sigma-1 receptor (sigma1R) is a non-opioid membrane receptor, which responds to a diverse array of synthetic ligands to exert various pharmacological effects. Meanwhile, candidates for endogenous ligands of sigma1R have also been identified. However, how endogenous ligands bind to sigma1R remains unknown. Here, we present crystal structures of sigma1R from Xenopus laevis (xlsigma1R) bound to two endogenous neurosteroid ligands, progesterone (a putative antagonist) and dehydroepiandrosterone sulfate (DHEAS) (a putative agonist), at 2.15-3.09 A resolutions. Both neurosteroids bind to a similar location in xlsigma1R mainly through hydrophobic interactions, but surprisingly, with opposite binding orientations. DHEAS also forms hydrogen bonds with xlsigma1R, whereas progesterone interacts indirectly with the receptor through water molecules near the binding site. Binding analyses are consistent with the xlsigma1R-neurosteroid complex structures. Furthermore, molecular dynamics simulations and structural data reveal a potential water entry pathway. Our results provide insight into binding of two endogenous neurosteroid ligands to sigma1R. Insight into binding of endogenous neurosteroid ligands to the sigma-1 receptor.,Fu C, Xiao Y, Zhou X, Sun Z Nat Commun. 2024 Jul 4;15(1):5619. doi: 10.1038/s41467-024-49894-7. PMID:38965213[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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