8qma: Difference between revisions

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-'''Unreleased structure'''
-The entry 8qma is ON HOLD
+==Structure of the plastid-encoded RNA polymerase complex (PEP) from Sinapis alba==
+<StructureSection load='8qma' size='340' side='right'caption='[[8qma]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[8qma]] is a 11 chain structure with sequence from [https://en.wikipedia.org/wiki/Sinapis_alba Sinapis alba]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8QMA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8QMA FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8qma FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8qma OCA], [https://pdbe.org/8qma PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8qma RCSB], [https://www.ebi.ac.uk/pdbsum/8qma PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8qma ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/RPOB_SINAL RPOB_SINAL]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Chloroplasts contain a dedicated genome that encodes subunits of the photosynthesis machinery. Transcription of photosynthesis genes is predominantly carried out by a plastid-encoded RNA polymerase (PEP), a nearly 1 MDa complex composed of core subunits with homology to eubacterial RNA polymerases (RNAPs) and at least 12 additional chloroplast-specific PEP-associated proteins (PAPs). However, the architecture of this complex and the functions of the PAPs remain unknown. Here, we report the cryo-EM structure of a 19-subunit PEP complex from Sinapis alba (white mustard). The structure reveals that the PEP core resembles prokaryotic and nuclear RNAPs but contains chloroplast-specific features that mediate interactions with the PAPs. The PAPs are unrelated to known transcription factors and arrange around the core in a unique fashion. Their structures suggest potential functions during transcription in the chemical environment of chloroplasts. These results reveal structural insights into chloroplast transcription and provide a framework for understanding photosynthesis gene expression.
-Authors:
+Structure of the multi-subunit chloroplast RNA polymerase.,do Prado PFV, Ahrens FM, Liebers M, Ditz N, Braun HP, Pfannschmidt T, Hillen HS Mol Cell. 2024 Mar 7;84(5):910-925.e5. doi: 10.1016/j.molcel.2024.02.003. Epub , 2024 Feb 29. PMID:38428434<ref>PMID:38428434</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 8qma" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Sinapis alba]]
+[[Category: Ahrens FM]]
+[[Category: Hillen HS]]
+[[Category: Pfannschmidt T]]
+[[Category: Do Prado PFV]]